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High Temperature and High Hydrostatic Pressure Cultivation, Transfer, and Filtration Systems for Investigating Deep Marine Microorganisms

hal.structure.identifierDepartment of Earth and Environmental Sciences [Troy, NY]
dc.contributor.authorOLIVER, Gina Carole
hal.structure.identifierDepartment of Earth and Environmental Sciences [Troy, NY]
dc.contributor.authorCARIO, Anaïs
hal.structure.identifierDepartment of Earth and Environmental Sciences [Troy, NY]
hal.structure.identifierRensselaer Astrobiology Research and Education Center
dc.contributor.authorROGERS, Karyn Lynne
dc.date.accessioned2022-10-12T08:52:44Z
dc.date.available2022-10-12T08:52:44Z
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/150300
dc.description.abstractEnHigh temperatures (HT) and high hydrostatic pressures (HHP) are characteristic of deep-sea hydrothermal vents and other deep crustal settings. These environments host vast and diverse microbial populations, yet only a small fraction of those populations have been successfully cultured. This is due, in part, to the difficulty of sampling while maintaining these in situ conditions and also replicating those high-temperature and high-pressure conditions in the laboratory. In an effort to facilitate more HT-HHP cultivation, we present two HT-HHP batch culture incubation systems for cultivating deep-sea vent and subsurface (hyper)thermophilic microorganisms. One HT-HHP system can be used for batch cultivation up to 110 MPa and 121°C, and requires sample decompression during subsampling. The second HT-HHP system can be used to culture microorganisms up to 100 MPa and 160°C with variable-volume, pressure-retaining vessels that negate whole-sample decompression during subsampling. Here, we describe how to build cost effective heating systems for these two types of high-pressure vessels, as well as the protocols for HT-HHP microbial batch cultivation in both systems. Additionally, we demonstrate HHP transfer between the variable-volume vessels, which has utility in sampling and enrichment without decompression, laboratory isolation experiments, as well as HHP filtration.
dc.language.isoen
dc.title.enHigh Temperature and High Hydrostatic Pressure Cultivation, Transfer, and Filtration Systems for Investigating Deep Marine Microorganisms
dc.typeDocument de travail - Pré-publication
dc.identifier.doi10.20944/preprints202104.0453.v1
dc.subject.halChimie/Matériaux
bordeaux.hal.laboratoriesInstitut de Chimie de la Matière Condensée de Bordeaux (ICMCB) - UMR 5026*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.institutionCNRS
hal.identifierhal-03338547
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03338547v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=OLIVER,%20Gina%20Carole&CARIO,%20Ana%C3%AFs&ROGERS,%20Karyn%20Lynne&rft.genre=preprint


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