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Oligonucleotide solid nucleolipid nanoparticles against antibiotic resistance of ESBL-producing bacteria

hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorNGUYEN, Phuoc Vinh
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorAUBRY, Clémentine
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorBOUDAOUD, Narimane
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorGAUBERT, Alexandra
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorLANGLOIS, Marie-Hélène
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMARCHIVIE, Mathieu
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorGAUDIN, Karen
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorARPIN, Corinne
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorBARTHÉLÉMY, Philippe
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorKAUSS, Tina
dc.date.issued2022
dc.identifier.issn1999-4923
dc.description.abstractEnAntibiotic resistance has become a major issue in the global healthcare system, notably in the case of Gram-negative bacteria. Recent advances in technology with oligonucleotides have an enormous potential for tackling this problem, providing their efficient intrabacterial delivery. The current work aimed to apply this strategy by using a novel nanoformulation consisting of DOTAU, a nucleolipid carrier, in an attempt to simultaneously deliver antibiotic and anti-resistance oligonucleotides. Ceftriaxone, a third-generation cephalosporin, was formulated with DOTAU to form an ion pair, and was then nanoprecipitated. The obtained solid nanocapsules were characterized using FT-IR, XRD, HPLC, TEM and DLS techniques and further functionalized by the anti-resistance ONα sequence. To obtain an optimal anti-resistance activity and encapsulation yield, both the formulation protocol and the concentration of ONα were optimized. As a result, monodispersed negatively charged nanoparticles of CFX–DOTAU-ONα with a molar ratio of 10:24:1 were obtained. The minimum inhibitory concentration of these nanoparticles on the resistant Escherichia coli strain was significantly reduced (by 75%) in comparison with that of non-vectorized ONα. All aforementioned results reveal that our nanoformulation can be considered as an efficient and relevant strategy for oligonucleotide intrabacterial delivery in the fight against antibiotic resistance
dc.language.isoen
dc.publisherMDPI
dc.subject.enoligonucleotides
dc.subject.enantibiotic resistance
dc.subject.ensolid nanoparticles
dc.subject.ennanocapsules
dc.subject.enESBL-producing E. coli
dc.subject.ennucleolipid
dc.subject.ennucleic acids
dc.subject.enCTX-M15 ß-lactamase
dc.title.enOligonucleotide solid nucleolipid nanoparticles against antibiotic resistance of ESBL-producing bacteria
dc.typeArticle de revue
dc.identifier.doi10.3390/pharmaceutics14020299
dc.subject.halSciences du Vivant [q-bio]/Sciences pharmaceutiques/Pharmacie galénique
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie
bordeaux.journalPharmaceutics
bordeaux.page299
bordeaux.volume14
bordeaux.issue2
bordeaux.peerReviewedoui
hal.identifierhal-03603893
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03603893v1
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