Afficher la notice abrégée

Study of the MR relaxation of microglia cells labeled with Gd-DTPA-bearing nanoparticles.

hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorRIBOT, Emeline Julie
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorMIRAUX, Sylvain
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorDELVILLE, Marie-Hélène
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBOUCHAUD, Véronique
hal.structure.identifierPôle Microscopie Electronique
dc.contributor.authorLACOMME, Sabrina
hal.structure.identifierPôle Microscopie Electronique
dc.contributor.authorGONTIER, Etienne
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBOUZIER-SORE, Anne-Karine
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorFRANCONI, Jean-Michel
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorTHIAUDIERE, Eric
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorVOISIN, Pierre
dc.date.issued2009
dc.identifier.issn1555-4309
dc.description.abstractEnTherapies involving cells as vehicles need to visualize in situ the trafficking of the cells concerned. This cellular imaging can be driven by cell contrast agent-based nanoparticle internalization and non-invasive MRI (magnetic resonance imaging) detection. Here, microglial cells, that would transport a suicide gene to a glioma, were incubated for different times, with various concentrations of silica nanoparticles on which numerous Gd-DTPA were grafted. The goal of this study was to investigate the repartition of cell-associated particles. MRI was used to quantitatively follow the particle uptake process. Fluorescence microscopy images showed that, although most of the nanoparticles were internalized, some remained adsorbed on the extracellular membrane surface. The cells were then submitted to various treatments: glycine to release bound nanoparticles and/or ultrasound to destroy the cell membranes. The R(1) relaxation rates were measured at 4.7 T. R(1) was maximal for 4 h of incubation, decreased after 8 h and remained stable for the 24 following hours. The magnetic resonance signal of ultrasonicated and glycine-treated cells made it possible to quantify the loss of bound nanoparticles after 8 h. Nevertheless, this release did not prevent cell detection since the internalized nanoparticles are enough concentrated to visualize the labeled cells even after 4 days of cell growth. These results highlight the compartmentalization of nanoparticles in microglia and the evolution of the MR signal of the labeled cells. This study could be of importance to interpret in vivo the MR signal changes that could occur after administration of such nanoparticle-labeled cells in therapeutic strategies.
dc.language.isoen
dc.publisherWiley
dc.title.enStudy of the MR relaxation of microglia cells labeled with Gd-DTPA-bearing nanoparticles.
dc.typeArticle de revue
dc.identifier.doi10.1002/cmmi.268
dc.subject.halChimie/Matériaux
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
dc.subject.halInformatique [cs]/Biotechnologie
bordeaux.journalContrast Media and Molecular Imaging
bordeaux.page109-117
bordeaux.volume4
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-00403016
hal.version1
hal.popularnon
hal.audienceInternationale
dc.subject.itMicroglia
dc.subject.itCell
dc.subject.itNanoparticles
dc.subject.itContrast agent
dc.subject.itMRI
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00403016v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Contrast%20Media%20and%20Molecular%20Imaging&rft.date=2009&rft.volume=4&rft.issue=3&rft.spage=109-117&rft.epage=109-117&rft.eissn=1555-4309&rft.issn=1555-4309&rft.au=RIBOT,%20Emeline%20Julie&MIRAUX,%20Sylvain&DELVILLE,%20Marie-H%C3%A9l%C3%A8ne&BOUCHAUD,%20V%C3%A9ronique&LACOMME,%20Sabrina&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée