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Nanoparticle phagocytosis and cellular stress: involvement in cellular imaging and in gene therapy against glioma.

hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBOUZIER-SORE, Anne-Karine
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorRIBOT, Emeline
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBOUCHAUD, Véronique
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorMIRAUX, Sylvain
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorDUGUET, Etienne
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMORNET, Stéphane
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorCLOFENT-SANCHEZ, Gisèle
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorFRANCONI, Jean-Michel
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorVOISIN, Pierre
dc.date.issued2010
dc.identifier.issn0952-3480
dc.description.abstractEnIn gene therapy against glioma, targeting tumoral tissue is not an easy task. We used the tumor infiltrating property of microglia in this study. These cells are well adapted to this therapy since they can phagocyte nanoparticles and allow their visualization by MRI. Indeed, while many studies have used transfected microglia containing a suicide gene and other internalized nanoparticles to visualize microglia, none have combined both approaches during gene therapy. Microglia cells were transfected with the TK-GFP gene under the control of the HSP(70) promoter. First, the possible cellular stress induced by nanoparticle internalization was checked to avoid a non-specific activation of the suicide gene. Then, MR images were obtained on tubes containing microglia loaded with superparamagnetic nanoparticles (VUSPIO) to characterize their MR properties, as well as their potential to track cells in vivo. VUSPIO were efficiently internalized by microglia, were found non-toxic and their internalization did not induce any cellular stress. VUSPIO relaxivity r(2) was 224 mM(-1).s(-1). Such results could generate a very high contrast between loaded and unloaded cells on T(2)-weighted images. The intracellular presence of VUSPIO does not prevent suicide gene activity, since TK is expressed in vitro and functional in vivo. It allows MRI detection of gene modified macrophages during cell therapy strategies.
dc.language.isoen
dc.publisherWiley
dc.title.enNanoparticle phagocytosis and cellular stress: involvement in cellular imaging and in gene therapy against glioma.
dc.typeArticle de revue
dc.identifier.doi10.1002/nbm.1434
dc.subject.halChimie/Matériaux
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
dc.subject.halInformatique [cs]/Biotechnologie
bordeaux.journalNMR in Biomedicine
bordeaux.page88-96
bordeaux.volume23
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-00466418
hal.version1
hal.popularnon
hal.audienceInternationale
dc.subject.itMicroglia
dc.subject.itSuperparamagnetic contrast agent
dc.subject.itMRI
dc.subject.itGene therapy
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00466418v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=NMR%20in%20Biomedicine&rft.date=2010&rft.volume=23&rft.issue=1&rft.spage=88-96&rft.epage=88-96&rft.eissn=0952-3480&rft.issn=0952-3480&rft.au=BOUZIER-SORE,%20Anne-Karine&RIBOT,%20Emeline&BOUCHAUD,%20V%C3%A9ronique&MIRAUX,%20Sylvain&DUGUET,%20Etienne&rft.genre=article


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