Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth
| dc.rights.license | open | en_US |
| hal.structure.identifier | Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB] | |
| dc.contributor.author | DOS-SANTOS CARVALHO, Steve | |
| hal.structure.identifier | Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB] | |
| dc.contributor.author | MOREAU, Maite M. | |
| hal.structure.identifier | Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB] | |
| dc.contributor.author | HIEN, Yeri Esther | |
| hal.structure.identifier | Interdisciplinary Institute for Neuroscience [Bordeaux] [IINS] | |
| dc.contributor.author | GARCIA, Michael | |
| hal.structure.identifier | Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB] | |
| dc.contributor.author | AUBAILLY, Nathalie | |
| dc.contributor.author | HENDERSON, Deborah J. | |
| hal.structure.identifier | Interdisciplinary Institute for Neuroscience [Bordeaux] [IINS] | |
| dc.contributor.author | STUDER, Vincent | |
| hal.structure.identifier | Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB] | |
| dc.contributor.author | SANS, Nathalie | |
| hal.structure.identifier | Interdisciplinary Institute for Neuroscience [Bordeaux] [IINS] | |
| dc.contributor.author | THOUMINE, Olivier | |
| hal.structure.identifier | Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB] | |
| dc.contributor.author | MONTCOUQUIOL, Mireille | |
| dc.date.accessioned | 2022-07-12T12:47:02Z | |
| dc.date.available | 2022-07-12T12:47:02Z | |
| dc.date.issued | 2020-01-07 | |
| dc.identifier.issn | 2050-084X | en_US |
| dc.identifier.uri | oai:crossref.org:10.7554/elife.51822 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/140441 | |
| dc.description.abstractEn | Dynamic mechanical interactions between adhesion complexes and the cytoskeleton are essential for axon outgrowth and guidance. Whether planar cell polarity (PCP) proteins, which regulate cytoskeleton dynamics and appear necessary for some axon guidance, also mediate interactions with membrane adhesion is still unclear. Here we show that Vangl2 controls growth cone velocity by regulating the internal retrograde actin flow in an N-cadherin-dependent fashion. Single molecule tracking experiments show that the loss of Vangl2 decreased fast-diffusing N-cadherin membrane molecules and increased confined N-cadherin trajectories. Using optically manipulated N-cadherin-coated microspheres, we correlated this behavior to a stronger mechanical coupling of N-cadherin with the actin cytoskeleton. Lastly, we show that the spatial distribution of Vangl2 within the growth cone is selectively affected by an N-cadherin-coated substrate. Altogether, our data show that Vangl2 acts as a negative regulator of axonal outgrowth by regulating the strength of the molecular clutch between N-cadherin and the actin cytoskeleton. | |
| dc.description.sponsorship | Signalisation de la polarité cellulaire planaire dans la plasticité synaptique morphofonctionnelle des circuits hippocampiques | en_US |
| dc.description.sponsorship | Bordeaux Region Aquitaine Initiative for Neuroscience - ANR-10-LABX-0043 | en_US |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.source | crossref | |
| dc.subject.en | PCP | |
| dc.subject.en | Adhesion | |
| dc.subject.en | Cell biology | |
| dc.subject.en | Cytoskeleton | |
| dc.subject.en | Developmental biology | |
| dc.subject.en | Mouse | |
| dc.subject.en | Neuron | |
| dc.subject.en | Rat | |
| dc.subject.en | Super-resolution | |
| dc.subject.en | Vangl2 | |
| dc.title.en | Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.7554/elife.51822 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC] | en_US |
| dc.identifier.pubmed | 31909712 | en_US |
| dc.description.sponsorshipEurope | EU Framework Programme Horizon 2020 | en_US |
| bordeaux.journal | eLife | en_US |
| bordeaux.volume | 9 | en_US |
| bordeaux.hal.laboratories | Neurocentre Magendie - UMR-S 1215 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INSERM | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.identifier.funderID | Fondation pour la Recherche Médicale | en_US |
| bordeaux.identifier.funderID | Conseil Régional Aquitaine | en_US |
| bordeaux.identifier.funderID | British Heart Foundation | en_US |
| bordeaux.import.source | dissemin | |
| hal.export | false | |
| workflow.import.source | dissemin | |
| dc.rights.cc | CC BY | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=eLife&rft.date=2020-01-07&rft.volume=9&rft.eissn=2050-084X&rft.issn=2050-084X&rft.au=DOS-SANTOS%20CARVALHO,%20Steve&MOREAU,%20Maite%20M.&HIEN,%20Yeri%20Esther&GARCIA,%20Michael&AUBAILLY,%20Nathalie&rft.genre=article |
