Micro-pixe characterization of different skin models
Language
en
Article de revue
This item was published in
X-Ray Spectrometry, X-ray Spectrometry, X-ray Spectrometry, 2004-06-04, Portoroz. 2005, vol. 34, p. 381-388
Wiley
English Abstract
Because of the large natural inter-individual variability and of some difficulties in obtaining material from surgery or from healthy volunteers, the use of human native skin in experimentation comes up against fierce ...Read more >
Because of the large natural inter-individual variability and of some difficulties in obtaining material from surgery or from healthy volunteers, the use of human native skin in experimentation comes up against fierce competition from experimental models, such as reconstituted epidermis or human skin grafted in animals. In this study, micro-PIXE was used in association with other ion beam microanalysis techniques to characterize different epidermis models on a microscopic scale. The ionic species were found to be highly compartmentalized in the different strata of human skin with a distribution that can be explained in the frame of the homeostatic barrier function. Reconstituted epidermis obtained from airlifted culture, epidermis of mice foot sole and human forehead skin grafts transplanted into mice are the models investigated and compared with human native skin. Very similar inorganic ion patterns were observed in all epidermis, suggesting comparable permeability barrier mechanisms and validating their use as alternative approaches to native skin experimentation. In the near future, we plan to use some of these models in penetration studies and for investigating effects of exposure to different environmental stresses.Read less <
Origin
Hal imported