Comparative study between reconstructed and native human epidermis using nuclear microscopy
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | YNSA, M.D. | |
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | GONTIER, E. | |
| hal.structure.identifier | Institut de Recherche Pierre FABRE [INSTITUT DE RECHERCHE PIERRE FABRE] | |
| dc.contributor.author | MAVON, A. | |
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | MORETTO, P. | |
| hal.structure.identifier | SkinEthic Laboratories [SKINETHIC LABORATORIES] | |
| dc.contributor.author | ROSDY, M. | |
| dc.contributor.editor | Dirk E. Boerma, Aurelio Climent-Font and Miguel Ángel Respaldiza | |
| dc.date.issued | 2006 | |
| dc.date.conference | 2005-06-26 | |
| dc.identifier.issn | 0168-583X | |
| dc.description.abstractEn | The physiological status of native skin is suffering from large inter-individual variations, especially in terms of inorganic ions content. For this reason, together with the advent of ethic laws on animal experimentation, reconstructed skin or epidermis models are extensively employed nowadays in penetration studies for cosmetic or pharmacological applications. It has been already verified that reconstructed human epidermis (RHE) has similar physiological mechanisms to native human skin, but until now, there are few studies where the elemental concentrations of both skins, reconstructed and native, are compared. In this work, freeze-dried thin sections of human native skin obtained from surgery have been characterized using PIXE, RBS and STIM at the CENBG nuclear microprobe. RHE samples were treated and analyzed in the same conditions for comparison. The combination of the different imaging and analysis techniques made possible a clear delimitation and identification of skin ultrastructure. The elemental concentrations of P, S, Cl, K and Ca were measured in the different strata. For both skins, concentrations have been compared and significant differences in terms of elemental concentrations have been determined using statistical approaches. Similar physiological characteristics were pointed out in both skin models, in particular the Ca gradient presumably involved in the regulation of the barrier effect. | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.source.title | Nuclear Instruments and Methods in Physics Research B | |
| dc.subject.en | Micro-PIXE | |
| dc.subject.en | Micro-RBS | |
| dc.subject.en | STIM | |
| dc.subject.en | Skin models | |
| dc.subject.en | Native skin | |
| dc.subject.en | Epidermis | |
| dc.title.en | Comparative study between reconstructed and native human epidermis using nuclear microscopy | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1016/j.nimb.2006.03.123 | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Physique Médicale [physics.med-ph] | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Dermatologie | |
| bordeaux.journal | Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms | |
| bordeaux.page | 710-714 | |
| bordeaux.volume | 249 | |
| bordeaux.country | ES | |
| bordeaux.title.proceeding | Nuclear Instruments and Methods in Physics Research B | |
| bordeaux.conference.city | Seville | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | in2p3-00090050 | |
| hal.version | 1 | |
| hal.conference.end | 2005-07-01 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//in2p3-00090050v1 | |
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