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Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epithelia.

hal.structure.identifierService Interdisciplinaire sur les Systèmes Moléculaires et les Matériaux (ex SCM) [SIS2M UMR 3299]
dc.contributor.authorBRUN, Emilie
hal.structure.identifierInflammation intestinale pathologique de l'enfant
hal.structure.identifierCentre de Physiopathologie Toulouse Purpan [CPTP]
dc.contributor.authorBARREAU, Frédérick
hal.structure.identifierEuropean Synchrotron Radiation Facility [ESRF]
dc.contributor.authorVERONESI, Giulia
hal.structure.identifierEuropean Synchrotron Radiation Facility [ESRF]
dc.contributor.authorFAYARD, Barbara
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSORIEUL, Stéphanie
hal.structure.identifierLaboratoire de Chimie de la Matière Condensée de Paris [LCMCP]
dc.contributor.authorCHANÉAC, Corinne
hal.structure.identifierLaboratoire de Spectrométrie de Masse BioOrganique [Strasbourg] [LSMBO]
dc.contributor.authorCARAPITO, Christine
hal.structure.identifierLaboratoire de Chimie et Biologie des Métaux [LCBM - UMR 5249]
dc.contributor.authorRABILLOUD, Thierry
hal.structure.identifierInstitut de Biologie et de Technologies de Saclay [IBITECS]
dc.contributor.authorMABONDZO, Aloïse
hal.structure.identifierLaboratoire Francis PERRIN [LFP - URA 2453]
dc.contributor.authorHERLIN-BOIME, Nathalie
hal.structure.identifierInstitut Nanosciences et Cryogénie [INAC]
dc.contributor.authorCARRIÈRE, Marie
dc.date.issued2014
dc.identifier.issn1743-8977
dc.description.abstractEnBACKGROUND: TiO2 particles are commonly used as dietary supplements and may contain up to 36% of nano-sized particles (TiO2-NPs). Still impact and translocation of NPs through the gut epithelium is poorly documented. RESULTS: We show that, in vivo and ex vivo, agglomerates of TiO2-NPs cross both the regular ileum epithelium and the follicle-associated epithelium (FAE) and alter the paracellular permeability of the ileum and colon epithelia. In vitro, they accumulate in M-cells and mucus-secreting cells, much less in enterocytes. They do not cause overt cytotoxicity or apoptosis. They translocate through a model of FAE only, but induce tight junctions remodeling in the regular ileum epithelium, which is a sign of integrity alteration and suggests paracellular passage of NPs. Finally we prove that TiO2-NPs do not dissolve when sequestered up to 24 h in gut cells. CONCLUSIONS: Taken together these data prove that TiO2-NPs would possibly translocate through both the regular epithelium lining the ileum and through Peyer's patches, would induce epithelium impairment, and would persist in gut cells where they would possibly induce chronic damage.
dc.language.isoen
dc.publisherBioMed Central
dc.subject.enTitanium dioxide
dc.subject.enNanoparticle
dc.subject.enIngestion
dc.subject.enTranslocation
dc.subject.enDissolution
dc.subject.enAccumulation
dc.subject.enGut
dc.subject.enToxicity
dc.subject.enM-cells
dc.subject.enParacellular
dc.title.enTitanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epithelia.
dc.typeArticle de revue
dc.identifier.doi10.1186/1743-8977-11-13
dc.subject.halSciences du Vivant [q-bio]/Santé publique et épidémiologie
bordeaux.journalParticle and Fibre Toxicology
bordeaux.page13
bordeaux.volume11
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierinserm-00971511
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-00971511v1
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