Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epithelia.
BRUN, Emilie
Service Interdisciplinaire sur les Systèmes Moléculaires et les Matériaux (ex SCM) [SIS2M UMR 3299]
Service Interdisciplinaire sur les Systèmes Moléculaires et les Matériaux (ex SCM) [SIS2M UMR 3299]
BARREAU, Frédérick
Inflammation intestinale pathologique de l'enfant
Centre de Physiopathologie Toulouse Purpan [CPTP]
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Inflammation intestinale pathologique de l'enfant
Centre de Physiopathologie Toulouse Purpan [CPTP]
BRUN, Emilie
Service Interdisciplinaire sur les Systèmes Moléculaires et les Matériaux (ex SCM) [SIS2M UMR 3299]
Service Interdisciplinaire sur les Systèmes Moléculaires et les Matériaux (ex SCM) [SIS2M UMR 3299]
BARREAU, Frédérick
Inflammation intestinale pathologique de l'enfant
Centre de Physiopathologie Toulouse Purpan [CPTP]
< Reduce
Inflammation intestinale pathologique de l'enfant
Centre de Physiopathologie Toulouse Purpan [CPTP]
Language
en
Article de revue
This item was published in
Particle and Fibre Toxicology. 2014, vol. 11, n° 1, p. 13
BioMed Central
English Abstract
BACKGROUND: TiO2 particles are commonly used as dietary supplements and may contain up to 36% of nano-sized particles (TiO2-NPs). Still impact and translocation of NPs through the gut epithelium is poorly documented. ...Read more >
BACKGROUND: TiO2 particles are commonly used as dietary supplements and may contain up to 36% of nano-sized particles (TiO2-NPs). Still impact and translocation of NPs through the gut epithelium is poorly documented. RESULTS: We show that, in vivo and ex vivo, agglomerates of TiO2-NPs cross both the regular ileum epithelium and the follicle-associated epithelium (FAE) and alter the paracellular permeability of the ileum and colon epithelia. In vitro, they accumulate in M-cells and mucus-secreting cells, much less in enterocytes. They do not cause overt cytotoxicity or apoptosis. They translocate through a model of FAE only, but induce tight junctions remodeling in the regular ileum epithelium, which is a sign of integrity alteration and suggests paracellular passage of NPs. Finally we prove that TiO2-NPs do not dissolve when sequestered up to 24 h in gut cells. CONCLUSIONS: Taken together these data prove that TiO2-NPs would possibly translocate through both the regular epithelium lining the ileum and through Peyer's patches, would induce epithelium impairment, and would persist in gut cells where they would possibly induce chronic damage.Read less <
English Keywords
Titanium dioxide
Nanoparticle
Ingestion
Translocation
Dissolution
Accumulation
Gut
Toxicity
M-cells
Paracellular
Origin
Hal imported