Show simple item record

hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorLE TREQUESSER, Quentin
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorDEVÈS, Guillaume
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorSAEZ, Gladys
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorDAUDIN, Laurent
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorBARBERET, Philippe
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorMICHELET, Claire
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorDELVILLE, Marie-Hélène
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorSEZNEC, Hervé
dc.date.issued2014
dc.identifier.issn0003-2700
dc.description.abstractEnAssessing in situ nanoparticles (NPs) internalization at the level of a single cell is a difficult but critical task due to their potential use in nanomedicine. One of the main actual challenges is to control the number of internalized NPs per cell. To in situ detect, track, and above all quantify NPs in a single cell, we propose an approach based on a multimodal correlative microscopy (MCM), via the complementarity of three imaging techniques: fluorescence microscopy (FM), scanning electron microscopy (SEM), and ion beam analysis (IBA). This MCM was performed on single targeted individual primary human foreskin keratinocytes (PHFK) cells cultured and maintained on a specifically designed sample holder, to probe either dye-modified or bare NPs. The data obtained by both FM and IBA on dye-modified NPs were strongly correlated in terms of detection, tracking, and colocalization of fluorescence and metal detection. IBA techniques should therefore open a new field concerning specific studies on bare NPs and their toxicological impact on cells. Complementarity of SEM and IBA analyses provides surface (SEM) and in depth (IBA) information on the cell morphology as well as on the exact localization of the NPs. Finally, IBA not only provides in a single cell the in situ quantification of exogenous elements (NPs) but also that all the other endogenous elements and the subsequent variation of their homeostasis. This unique feature opens further insights in dose-dependent response analyses and adds the perspective of a better understanding of NPs behavior in biological specimens for toxicology or nanomedicine purposes.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.typeArticle de revue
dc.identifier.doi10.1021/ac501318c
dc.subject.halChimie/Matériaux
dc.subject.halChimie/Chimie analytique
bordeaux.journalAnalytical Chemistry
bordeaux.page7311-7319
bordeaux.volume86
bordeaux.issue15
bordeaux.peerReviewedoui
hal.identifierhal-01058969
hal.version1
hal.popularnon
hal.audienceInternationale
dc.title.itSingle cell in situ detection and quantification of metal oxide nanoparticles using multimodal correlative microscopy.
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01058969v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analytical%20Chemistry&rft.date=2014&rft.volume=86&rft.issue=15&rft.spage=7311-7319&rft.epage=7311-7319&rft.eissn=0003-2700&rft.issn=0003-2700&rft.au=LE%20TREQUESSER,%20Quentin&DEV%C3%88S,%20Guillaume&SAEZ,%20Gladys&DAUDIN,%20Laurent&BARBERET,%20Philippe&rft.genre=article


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record