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Simulation of early DNA damage after the irradiation of a fibroblast cell nucleus using Geant4-DNA

hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorMEYLAN, Sylvain
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorINCERTI, Sébastien
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorKARAMITROS, Mathieu
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorTANG, Nicolas
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorBUENO, Marta
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorCLAIRAND, Isabelle
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorVILLAGRASA, Carmen
dc.date.issued2017
dc.identifier.issn2045-2322
dc.description.abstractEnIn order to improve the understanding of the mechanisms involved in the generation of early DNA damage, a new calculation chain based on the Geant4-DNA toolkit was developed. This work presents for the first time the simulation of the physical, physicochemical and chemical stages of early radiation damage at the scale of an entire human genome (fibroblast, male) and using Geant4-DNA models. The DnaFabric software was extended to generate and export this nucleus model to a text file with a specific format that can be read by Geant4 user applications. This calculation chain was used to simulate the irradiation of the nucleus by primary protons of different energies (0,5; 0,7; 0,8; 1; 1,5; 2; 3; 4; 5; 10; 20 MeV) and the results, in terms of DNA double strand breaks, agree with experimental data found in the literature (pulsed field electrophoresis technique). These results show that the simulation is consistent and that its parameters are well balanced. Among the different parameters that can be adjusted, our results demonstrate that the criterion used to select direct strand break appears to have a very significant role on the final number of simulated double strand breaks.
dc.language.isoen
dc.publisherNature Publishing Group
dc.title.enSimulation of early DNA damage after the irradiation of a fibroblast cell nucleus using Geant4-DNA
dc.typeArticle de revue
dc.identifier.doi10.1038/s41598-017-11851-4
dc.subject.halPhysique [physics]
bordeaux.journalScientific Reports
bordeaux.page11923
bordeaux.volume7
bordeaux.peerReviewedoui
hal.identifierhal-01703528
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01703528v1
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