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Evidence of isotopic fractionation of natural uranium in cultured human cells

hal.structure.identifierCEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) [CEA-DES (ex-DEN)]
dc.contributor.authorPAREDES, Eduardo
hal.structure.identifierInstitut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
dc.contributor.authorAVAZERI, Emilie
hal.structure.identifierInstitut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
dc.contributor.authorMALARD, Veronique
hal.structure.identifierInstitut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
dc.contributor.authorVIDAUD, Claude
hal.structure.identifierCEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) [CEA-DES (ex-DEN)]
dc.contributor.authorREILLER, Pascal E.
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorORTEGA, Richard
hal.structure.identifierCEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) [CEA-DES (ex-DEN)]
dc.contributor.authorNONELL, Anthony
hal.structure.identifierCEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) [CEA-DES (ex-DEN)]
dc.contributor.authorISNARD, Hélène
hal.structure.identifierCEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) [CEA-DES (ex-DEN)]
dc.contributor.authorCHARTIER, Frédéric
hal.structure.identifierCEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) [CEA-DES (ex-DEN)]
dc.contributor.authorBRESSON, Carole
dc.date.issued2016
dc.description.abstractEnThe study of the isotopic fractionation of endogen elements and toxic heavy metals in living organisms for biomedical applications, and for metabolic and toxicological studies, is a cutting-edge research topic. This paper shows that human neuroblastoma cells incorporated small amounts of uranium (U) after exposure to 10 µM natural U, with preferential uptake of the 235U isotope with regard to 238U. Efforts were made to develop and then validate a procedure for highly accurate n(238U)/n(235U) determinations in microsamples of cells. We found that intracellular U is enriched in 235U by 0.38 ± 0.13‰ (2σ, n = 7) relative to the exposure solutions. These in vitro experiments provide clues for the identification of biological processes responsible for uranium isotopic fractionation and link them to potential U incorporation pathways into neuronal cells. Suggested incorporation processes are a kinetically controlled process, such as facilitated transmembrane diffusion, and the uptake through a high-affinity uranium transport protein involving the modification of the uranyl (UO22+) coordination sphere. These findings open perspectives on the use of isotopic fractionation of metals in cellular models, offering a probe to track uptake/transport pathways and to help decipher associated cellular metabolic processes.
dc.language.isoen
dc.title.enEvidence of isotopic fractionation of natural uranium in cultured human cells
dc.typeArticle de revue
dc.identifier.doi10.1073/pnas.1610885113
dc.subject.halPhysique [physics]
bordeaux.journalProc.Nat.Acad.Sci
bordeaux.page14007-14012
bordeaux.volume113
bordeaux.issue49
bordeaux.peerReviewedoui
hal.identifierhal-01758782
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01758782v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Proc.Nat.Acad.Sci&rft.date=2016&rft.volume=113&rft.issue=49&rft.spage=14007-14012&rft.epage=14007-14012&rft.au=PAREDES,%20Eduardo&AVAZERI,%20Emilie&MALARD,%20Veronique&VIDAUD,%20Claude&REILLER,%20Pascal%20E.&rft.genre=article


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