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Plant cell nucleolus as a hot spot for iron.

hal.structure.identifierBiochimie et Physiologie Moléculaire des Plantes [BPMP]
dc.contributor.authorROSCHZTTARDTZ, Hannetz
hal.structure.identifierBiochimie et Physiologie Moléculaire des Plantes [BPMP]
dc.contributor.authorGRILLET, Louis
hal.structure.identifierChimie Nucléaire Analytique et Bio-environnementale [CNAB]
dc.contributor.authorISAURE, M.-P.
hal.structure.identifierBiochimie et Physiologie Moléculaire des Plantes [BPMP]
dc.contributor.authorCONÉJÉRO, Geneviève
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorORTEGA, Richard
hal.structure.identifierBiochimie et Physiologie Moléculaire des Plantes [BPMP]
dc.contributor.authorCURIE, Catherine
hal.structure.identifierBiochimie et Physiologie Moléculaire des Plantes [BPMP]
dc.contributor.authorMARI, Stéphane
dc.date.issued2011-08-12
dc.identifier.issn0021-9258
dc.description.abstractEnMany central metabolic processes require iron as a cofactor and take place in specific subcellular compartments such as the mitochondrion or the chloroplast. Proper iron allocation in the different organelles is thus critical to maintain cell function and integrity. To study the dynamics of iron distribution in plant cells, we have sought to identify the different intracellular iron pools by combining three complementary imaging approaches, histochemistry, micro particle-induced x-ray emission, and synchrotron radiation micro X-ray fluorescence. Pea (Pisum sativum) embryo was used as a model in this study because of its large cell size and high iron content. Histochemical staining with ferrocyanide and diaminobenzidine (Perls/diaminobenzidine) strongly labeled a unique structure in each cell, which co-labeled with the DNA fluorescent stain DAPI, thus corresponding to the nucleus. The unexpected presence of iron in the nucleus was confirmed by elemental imaging using micro particle-induced x-ray emission. X-ray fluorescence on cryo-sectioned embryos further established that, quantitatively, the iron concentration found in the nucleus was higher than in the expected iron-rich organelles such as plastids or vacuoles. Moreover, within the nucleus, iron was particularly accumulated in a subcompartment that was identified as the nucleolus as it was shown to transiently disassemble during cell division. Taken together, our data uncover an as yet unidentified although abundant iron pool in the cell, which is located in the nuclei of healthy, actively dividing plant tissues. This result paves the way for the discovery of a novel cellular function for iron related to nucleus/nucleolus-associated processes.
dc.language.isoen
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dc.rights.urihttp://hal.archives-ouvertes.fr/licences/copyright/
dc.subject.enIron
dc.subject.enFe
dc.subject.endynamics of Fe
dc.subject.enFerro cyanide
dc.subject.endiaminobenzidine
dc.subject.en[mu]PIXE. X-Ray Fluorescence
dc.subject.enFe concentration
dc.subject.ennucleus
dc.subject.ennucleus/nucleolus-associated
dc.title.enPlant cell nucleolus as a hot spot for iron.
dc.typeArticle de revue
dc.identifier.doi10.1074/jbc.C111.269720
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalJournal of Biological Chemistry
bordeaux.page27863-6
bordeaux.volume286
bordeaux.issue32
bordeaux.peerReviewedoui
hal.identifierhal-00623165
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00623165v1
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