Phosphoinositides containing stearic acid are required for interaction between Rho GTPases and the exocyst to control the late steps of polarized exocytosis
| dc.rights.license | open | en_US |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | LAQUEL-ROBERT, Patricia
IDREF: 31266924 | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | TESTET, Eric
IDREF: 03328184X | |
| hal.structure.identifier | Acides Nucléiques : Régulations Naturelle et Artificielle [ARNA] | |
| dc.contributor.author | TUPHILE, Karine | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | CULLIN, Christophe
ORCID: 0000-0003-4110-4677 IDREF: 85920959 | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | FOUILLEN, Laetitia
ORCID: 0000-0002-1204-9296 IDREF: 136936385 | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | BESSOULE, Jean-Jacques
ORCID: 0000-0002-5717-5210 IDREF: 072151846 | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | DOIGNON, Francois
ORCID: 0000-0002-9007-2408 IDREF: 097088439 | |
| dc.date.accessioned | 2022-02-09T15:51:52Z | |
| dc.date.available | 2022-02-09T15:51:52Z | |
| dc.date.issued | 2022-02-01 | |
| dc.identifier.issn | 1600-0854 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/124693 | |
| dc.description.abstractEn | Cell polarity is achieved by regulators such as small G proteins, exocyst members and phosphoinositides, with the latter playing a key role when bound to the exocyst proteins Sec3p and Exo70p, and Rho GTPases. This ensures asymmetric growth via the routing of proteins and lipids to the cell surface using actin cables. Previously, using a yeast mutant for a lysophosphatidylinositol acyl transferase encoded by the PSI1 gene, we demonstrated the role of stearic acid in the acyl chain of phosphoinositides in cytoskeletal organization and secretion. Here, we use a genetic approach to characterize the effect on late steps of the secretory pathway. The constitutive overexpression of PSI1 in mutants affecting kinases involved in the phosphoinositide pathway demonstrated the role of molecular species containing stearic acid in bypassing a lack of phosphatidylinositol-4-phosphate (PI(4)P) at the plasma membrane, which is essential for the function of the Cdc42p module. Decreasing the levels of stearic acid-containing phosphoinositides modifies the environment of the actors involved in the control of late steps in the secretory pathway. This leads to decreased interactions between Exo70p and Sec3p, with Cdc42p, Rho1p and Rho3p, because of disruption of the GTP/GDP ratio of at least Rho1p and Rho3p GTPases, thereby preventing activation of the exocyst. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | exocyst | |
| dc.subject.en | phosphoinositides | |
| dc.subject.en | polarised exocytosis | |
| dc.subject.en | Rho GTPases | |
| dc.title.en | Phosphoinositides containing stearic acid are required for interaction between Rho GTPases and the exocyst to control the late steps of polarized exocytosis | |
| dc.title.alternative | Traffic | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1111/tra.12829 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire | en_US |
| dc.identifier.pubmed | 34908215 | en_US |
| bordeaux.journal | Traffic | en_US |
| bordeaux.page | 120-136 | en_US |
| bordeaux.volume | 23 | en_US |
| bordeaux.hal.laboratories | Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200 | en_US |
| bordeaux.issue | 2 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.institution | INSERM | |
| bordeaux.institution | Bordeaux INP | |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-03563439 | |
| hal.version | 1 | |
| hal.date.transferred | 2022-02-09T15:51:56Z | |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Traffic&rft.date=2022-02-01&rft.volume=23&rft.issue=2&rft.spage=120-136&rft.epage=120-136&rft.eissn=1600-0854&rft.issn=1600-0854&rft.au=LAQUEL-ROBERT,%20Patricia&TESTET,%20Eric&TUPHILE,%20Karine&CULLIN,%20Christophe&FOUILLEN,%20Laetitia&rft.genre=article |
