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Population dynamics and yeast diversity in early winemaking stages without sulfites revealed by three complementary approaches
dc.rights.license | open | en_US |
hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
dc.contributor.author | WINDHOLTZ, Sara | |
hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
dc.contributor.author | DUTILH, Lucie | |
hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
dc.contributor.author | LUCAS, Marine | |
hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
dc.contributor.author | MAUPEU, Julie | |
hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
dc.contributor.author | VALLET-COURBIN, Amelie | |
hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
dc.contributor.author | FARRIS, Laura | |
dc.contributor.author | COULON, Joana | |
hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
dc.contributor.author | MASNEUF POMAREDE, Isabelle
ORCID: 0000-0002-8806-8944 IDREF: 13239667X | |
dc.date.accessioned | 2022-01-05T15:05:50Z | |
dc.date.available | 2022-01-05T15:05:50Z | |
dc.date.issued | 2021-03-11 | |
dc.identifier.issn | 2076-3417 | en_US |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/124324 | |
dc.description.abstractEn | Nowadays, the use of sulfur dioxide (SO2 ) during the winemaking process is a controversial societal issue. In order to reduce its use, various alternatives are emerging, in particular bioprotection by adding yeasts, with different impacts on yeast microbiota in early winemaking stages. In this study, quantitative-PCR and metabarcoding high-throughput sequencing (HTS) were combined with MALDI-TOF-MS to monitor yeast population dynamic and diversity in the early stages of red winemaking process without sulfites and with bioprotection by Torulaspora delbrueckii and Metschnikowia pulcherrima addition. By using standard procedures for yeast protein extraction and a laboratory-specific database of wine yeasts, identification at species level of 95% of the isolates was successfully achieved by MALDI-TOF-MS, thus confirming that it is a promising method for wine yeast identification. The different approaches confirmed the implantation and the niche occupation of bioprotection leading to the decrease of fungal communities (HTS) and Hanseniaspora uvarum cultivable population (MALDI-TOF MS). Yeast and fungi diversity was impacted by stage of maceration and, to a lesser extent, by bioprotection and SO2, resulting in a modification of the nature and abundance of the operational taxonomic units (OTUs) diversity. | |
dc.language.iso | EN | en_US |
dc.rights | Attribution 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
dc.subject.en | MALDI-TOF MS | |
dc.subject.en | Oenological laboratory specific database | |
dc.subject.en | Winemaking without SO2 | |
dc.subject.en | Bioprotection | |
dc.subject.en | Metabarcoding HTS | |
dc.subject.en | Quantitative-PCR | |
dc.title.en | Population dynamics and yeast diversity in early winemaking stages without sulfites revealed by three complementary approaches | |
dc.type | Article de revue | en_US |
dc.identifier.doi | 10.3390/app11062494 | en_US |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | en_US |
bordeaux.journal | Applied Sciences (Switzerland) | en_US |
bordeaux.volume | 11 | en_US |
bordeaux.hal.laboratories | Unité de Recherche Oenologie - EA 4577 | en_US |
bordeaux.issue | 6 | en_US |
bordeaux.institution | Université de Bordeaux | en_US |
bordeaux.institution | Bordeaux INP | en_US |
bordeaux.institution | INRAE | en_US |
bordeaux.peerReviewed | oui | en_US |
bordeaux.inpress | non | en_US |
hal.identifier | hal-03512959 | |
hal.version | 1 | |
hal.date.transferred | 2022-01-05T15:05:54Z | |
hal.export | true | |
dc.rights.cc | CC BY | en_US |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Applied%20Sciences%20(Switzerland)&rft.date=2021-03-11&rft.volume=11&rft.issue=6&rft.eissn=2076-3417&rft.issn=2076-3417&rft.au=WINDHOLTZ,%20Sara&DUTILH,%20Lucie&LUCAS,%20Marine&MAUPEU,%20Julie&VALLET-COURBIN,%20Amelie&rft.genre=article |