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Comparison of amniotic membrane versus the induced membrane for bone regeneration in long bone segmental defects using calcium phosphate cement loaded with BMP-2.

dc.rights.licenseopenen_US
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFENELON, Mathilde
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorETCHEBARNE, Marion
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, Robin
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorGRÉMARE, Agathe
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDURAND, Marlène
dc.contributor.authorSENTILHES, Loic
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorCATROS, Sylvain
dc.contributor.authorGINDRAUX, Florelle
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorL'HEUREUX, Nicolas
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, Jean-Christophe
ORCID: 0000-0001-7855-6437
dc.date.accessioned2021-12-21T08:40:01Z
dc.date.available2021-12-21T08:40:01Z
dc.date.issued2021-05-01
dc.identifier.issn1873-0191en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/124232
dc.description.abstractEnThanks to its biological properties, the human amniotic membrane (HAM) combined with a bone substitute could be a single-step surgical alternative to the two-step Masquelet induced membrane (IM) technique for regeneration of critical bone defects. However, no study has directly compared these two membranes. We first designed a 3D-printed scaffold using calcium phosphate cement (CPC). We assessed its suitability in vitro to support human bone marrow mesenchymal stromal cells (hBMSCs) attachment and osteodifferentiation. We then performed a rat femoral critical size defect to compare the two-step IM technique with a single-step approach using the HAM. Five conditions were compared. Group 1 was left empty. Group 2 received the CPC scaffold loaded with rh-BMP2 (CPC/BMP2). Group 3 and 4 received the CPC/BMP2 scaffold covered with lyophilized or decellularized/lyophilized HAM. Group 5 underwent a two- step induced membrane procedure with insertion of a polymethylmethacrylate (PMMA) spacer followed by, after 4 weeks, its replacement with the CPC/BMP2 scaffold wrapped in the IM. Micro-CT and histomorphometric analysis were performed after six weeks. Results showed that the CPC scaffold supported the proliferation and osteodifferentiation of hBMSCs in vitro. In vivo, the CPC/BMP2 scaffold very efficiently induced bone formation and led to satisfactory healing of the femoral defect, in a single-step, without autograft or the need for any membrane covering. In this study, there was no difference between the two-step induced membrane procedure and a single step approach. However, the results indicated that none of the tested membranes further enhanced bone healing compared to the CPC/BMP2 group.
dc.language.isoENen_US
dc.subject.enAmnion
dc.subject.enAnimals
dc.subject.enBone Cements
dc.subject.enBone Regeneration
dc.subject.enCalcium Phosphates
dc.subject.enOsteogenesis
dc.subject.enRats
dc.subject.enTissue Scaffolds
dc.title.enComparison of amniotic membrane versus the induced membrane for bone regeneration in long bone segmental defects using calcium phosphate cement loaded with BMP-2.
dc.title.alternativeMater Sci Eng C Mater Biol Applen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.msec.2021.112032en_US
dc.subject.halSciences du Vivant [q-bio]/Biotechnologiesen_US
dc.identifier.pubmed33947534en_US
bordeaux.journalMaterials Science and Engineering: Cen_US
bordeaux.page112032en_US
bordeaux.volume124en_US
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - UMR_S 1026en_US
bordeaux.institutionCNRSen_US
bordeaux.institutionINSERMen_US
bordeaux.institutionCHU de Bordeauxen_US
bordeaux.institutionInstitut Bergoniéen_US
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.identifierhal-03498545
hal.version1
hal.date.transferred2021-12-21T08:40:04Z
hal.exporttrue
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Materials%20Science%20and%20Engineering:%20C&rft.date=2021-05-01&rft.volume=124&rft.spage=112032&rft.epage=112032&rft.eissn=1873-0191&rft.issn=1873-0191&rft.au=FENELON,%20Mathilde&ETCHEBARNE,%20Marion&SIADOUS,%20Robin&GR%C3%89MARE,%20Agathe&DURAND,%20Marl%C3%A8ne&rft.genre=article


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