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Cell-assembled extracellular matrix (CAM) sheet production: Translation from using human to large animal cells.

dc.rights.licenseopenen_US
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorTORRES, Yoann
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorGLUAIS, Maude
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDA SILVA, Nicolas
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorREY, Sylvie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorGRÉMARE, Agathe
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorMAGNAN, Laure
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorKAWECKI, Fabien
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorL'HEUREUX, Nicolas
dc.date.accessioned2021-12-21T08:35:19Z
dc.date.available2021-12-21T08:35:19Z
dc.date.issued2021-01
dc.identifier.issn2041-7314en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/124226
dc.description.abstractEnWe have created entirely biological tissue-engineered vascular grafts (TEVGs) using sheets of cell-assembled extracellular matrix (CAM) produced by human fibroblasts . A large animal TEVG would allow long-term pre-clinical studies in a clinically relevant setting (graft size and allogeneic setting). Therefore, canine, porcine, ovine, and human skin fibroblasts were compared for their ability to form CAM sheets. Serum sourcing greatly influenced CAM production in a species-dependent manner. Ovine cells produced the most homogenous and strongest animal CAM sheets but remained ≈3-fold weaker than human sheets despite variations of serum, ascorbate, insulin, or growth factor supplementations. Key differences in cell growth dynamics, tissue development, and tissue architecture and composition were observed between human and ovine. This study demonstrates critical species-to-species differences in fibroblast behavior and how they pose a challenge when attempting to substitute animal cells for human cells during the development of tissue-engineered constructs that require long-term cultures.
dc.language.isoENen_US
dc.rightsAttribution-NonCommercial 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/us/*
dc.subject.ennatural polysaccharides
dc.subject.eninjectable matrix
dc.subject.enbone regeneration
dc.subject.enoral surgery
dc.title.enCell-assembled extracellular matrix (CAM) sheet production: Translation from using human to large animal cells.
dc.title.alternativeJ Tissue Engen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1177/2041731420978327en_US
dc.identifier.pubmed33633827en_US
bordeaux.journalJournal of Developmental Biology and Tissue Engineeringen_US
bordeaux.page2041731420978327en_US
bordeaux.volume12en_US
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - UMR_S 1026en_US
bordeaux.institutionCNRSen_US
bordeaux.institutionINSERMen_US
bordeaux.institutionCHU de Bordeauxen_US
bordeaux.institutionInstitut Bergoniéen_US
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.identifierinserm-03325055
hal.version1
hal.exportfalse
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Developmental%20Biology%20and%20Tissue%20Engineering&rft.date=2021-01&rft.volume=12&rft.spage=2041731420978327&rft.epage=2041731420978327&rft.eissn=2041-7314&rft.issn=2041-7314&rft.au=TORRES,%20Yoann&GLUAIS,%20Maude&DA%20SILVA,%20Nicolas&REY,%20Sylvie&GR%C3%89MARE,%20Agathe&rft.genre=article


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