GUILLAUMIE, Sabine; DECROOCQ, Stéphane; OLLAT, Nathalie; DELROT, Serge; GOMES, Eric; COOKSON, Sarah

doi:10.1186/s12870-020-2258-0

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GUILLAUMIE, Sabine

Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

DECROOCQ, Stéphane
Biologie du fruit et pathologie [BFP]

OLLAT, Nathalie

Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

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Ce document a été publié dans

BMC Plant Biology. 2020-01-29, vol. 20, n° 1, p. 43

Résumé en anglais

Grapevine is a crop of major economic importance, yet little is known about the regulation of shoot development in grapevine or other perennial fruits crops. Here we combine genetic and genomic tools to identify candidate genes regulating shoot development in Vitis spp. An F2 population from an interspecific cross between V. vinifera and V. riparia was phenotyped for shoot development traits, and three Quantitative Trait Loci (QTLs) were identified on linkage groups (LGs) 7, 14 and 18. Around 17% of the individuals exhibited a dwarfed phenotype. A transcriptomic study identified four candidate genes that were not expressed in dwarfed individuals and located within the confidence interval of the QTL on LG7. A deletion of 84,482 bp was identified in the genome of dwarfed plants, which included these four not expressed genes. One of these genes was VviCURLY LEAF (VviCLF), an orthologue of CLF, a regulator of shoot development in Arabidopsis thaliana. The phenotype of the dwarfed grapevine plants was similar to that of clf mutants of A. thaliana and orthologues of the known targets of CLF in A. thaliana were differentially expressed in the dwarfed plants. This suggests that CLF, a major developmental regulator in A. thaliana, also controls shoot development in grapevine.< Réduire

Mots clés

Arabidopsis Proteins

Chimera

Chromosome Mapping

Genes

Plant

Genome

Plant

Homeodomain Proteins

Phenotype

Plant Shoots

Quantitative Trait Loci

Transcriptome

Vitis

Mots clés en anglais

CURLY LEAF

Dwarfed phenotype

F2 population

Quantitative trait loci

Shoot development

Vitis interspecific cross