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Molecular and functional characterization of the SBP-box transcription factor SPL-CNR in tomato fruit ripening and cell death.

dc.rights.licenseopenen_US
dc.contributor.authorLAI, Tongfei
dc.contributor.authorWANG, Xiaohong
dc.contributor.authorYE, Bishun
dc.contributor.authorJIN, Mingfei
dc.contributor.authorCHEN, Weiwei
dc.contributor.authorWANG, Ying
dc.contributor.authorZHOU, Yingying
dc.contributor.authorBLANKS, Andrew M
dc.contributor.authorGU, Mei
dc.contributor.authorZHANG, Pengcheng
dc.contributor.authorZHANG, Xinlian
dc.contributor.authorLI, Chunyang
dc.contributor.authorWANG, Huizhong
dc.contributor.authorLIU, Yule
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGALLUSCI, Philippe
dc.contributor.authorTÖR, Mahmut
dc.contributor.authorHONG, Yiguo
dc.date.accessioned2020-09-10T14:55:47Z
dc.date.available2020-09-10T14:55:47Z
dc.date.issued2020-05-30
dc.identifier.issn1460-2431en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/11291
dc.description.abstractEnSlSPL-CNR, an SBP-box transcription factor (TF) gene residing at the epimutant Colourless non-ripening (Cnr) locus, is involved in tomato ripening. This epimutant provides a unique model to investigate the (epi)genetic basis of fruit ripening. Here we report that SlSPL-CNR is a nucleus-localized protein with a distinct monopartite nuclear localization signal (NLS). It consists of four consecutive residues ' 30KRKR33' at the N-terminus of the protein. Mutation of the NLS abolishes SlSPL-CNR's ability to localize in the nucleus. SlSPL-CNR comprises two zinc-finger motifs (ZFMs) within the C-terminal SBP-box domain. Both ZFMs contribute to zinc-binding activity. SlSPL-CNR can induce cell death in tomato and tobacco, dependent on its nuclear localization. However, the two ZFMs have differential impacts on SlSPL-CNR's induction of severe necrosis or mild necrotic ringspot. NLS and ZFM mutants cannot complement Cnr fruits to ripen. SlSPL-CNR interacts with SlSnRK1. Virus-induced SlSnRK1 silencing leads to reduction in expression of ripening-related genes and inhibits ripening in tomato. We conclude that SlSPL-CNR is a multifunctional protein that consists of a distinct monopartite NLS, binds to zinc, and interacts with SlSnRK1 to affect cell death and tomato fruit ripening.
dc.language.isoENen_US
dc.subject.enColourless non-ripening
dc.subject.enCell death
dc.subject.enSlSPL-CNR
dc.subject.enSlSnRK1
dc.subject.ennuclear localization signal
dc.subject.entomato (Solanum lycopersicum) fruit ripening
dc.subject.enzinc-finger motif
dc.titleMolecular and functional characterization of the SBP-box transcription factor SPL-CNR in tomato fruit ripening and cell death.
dc.title.alternativeJ Exp Boten_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1093/jxb/eraa067en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed32016417en_US
bordeaux.journalJournal of Experimental Botanyen_US
bordeaux.page2995-3011en_US
bordeaux.volume71en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.issue10en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.exportfalse
workflow.import.sourcepubmed
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