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Glycerol suppresses glucose consumption in trypanosomes through metabolic contest.

dc.rights.licenseopenen_US
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorALLMANN, Stefan
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorWARGNIES, Marion
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPLAZOLLES, Nicolas
dc.contributor.authorCAHOREAU, Edern
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBIRAN, Marc
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorMORAND, Pauline
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPINEDA, Erika
dc.contributor.authorKULYK, Hanna
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorASENCIO, Corinne
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorVILLAFRAZ, Oriana
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorRIVIÈRE, Loïc
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorTETAUD, Emmanuel
dc.contributor.authorROTUREAU, Brice
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorMOURIER, Arnaud
dc.contributor.authorPORTAIS, Jean-Charles
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBRINGAUD, Frédéric
dc.date.accessioned2021-10-06T06:35:24Z
dc.date.available2021-10-06T06:35:24Z
dc.date.issued2021-08-01
dc.identifier.issn1545-7885en_US
dc.identifier.otherhttps://figshare.com/articles/journal_contribution/Growth_curves_of_the_parental_WT_and_the_tetracycline-induced_sup_i_RNAi_i_sup_GK_i_cell_lines_maintained_in_the_presence_of_10_mM_glucose_and_glycerol_Glc_Glyc_10_mM_glucose_Glc_Glyc_10_mM_glycerol_Glc_Glyc_or_none_of_them_Glc_Glyc_/15166659
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/112555
dc.description.abstractEnMicroorganisms must make the right choice for nutrient consumption to adapt to their changing environment. As a consequence, bacteria and yeasts have developed regulatory mechanisms involving nutrient sensing and signaling, known as "catabolite repression," allowing redirection of cell metabolism to maximize the consumption of an energy-efficient carbon source. Here, we report a new mechanism named "metabolic contest" for regulating the use of carbon sources without nutrient sensing and signaling. Trypanosoma brucei is a unicellular eukaryote transmitted by tsetse flies and causing human African trypanosomiasis, or sleeping sickness. We showed that, in contrast to most microorganisms, the insect stages of this parasite developed a preference for glycerol over glucose, with glucose consumption beginning after the depletion of glycerol present in the medium. This "metabolic contest" depends on the combination of 3 conditions: (i) the sequestration of both metabolic pathways in the same subcellular compartment, here in the peroxisomal-related organelles named glycosomes; (ii) the competition for the same substrate, here ATP, with the first enzymatic step of the glycerol and glucose metabolic pathways both being ATP-dependent (glycerol kinase and hexokinase, respectively); and (iii) an unbalanced activity between the competing enzymes, here the glycerol kinase activity being approximately 80-fold higher than the hexokinase activity. As predicted by our model, an approximately 50-fold down-regulation of the GK expression abolished the preference for glycerol over glucose, with glucose and glycerol being metabolized concomitantly. In theory, a metabolic contest could be found in any organism provided that the 3 conditions listed above are met.
dc.language.isoENen_US
dc.title.enGlycerol suppresses glucose consumption in trypanosomes through metabolic contest.
dc.title.alternativePLoS Biolen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1371/journal.pbio.3001359en_US
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale
dc.identifier.pubmed34388147en_US
bordeaux.journalPlos Biologyen_US
bordeaux.pagee3001359en_US
bordeaux.volume19en_US
bordeaux.hal.laboratoriesCentre de Résonance Magnétique des Systèmes Biologiques (CRMSB) - UMR 5536en_US
bordeaux.issue8en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.exportfalse
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plos%20Biology&rft.date=2021-08-01&rft.volume=19&rft.issue=8&rft.spage=e3001359&rft.epage=e3001359&rft.eissn=1545-7885&rft.issn=1545-7885&rft.au=ALLMANN,%20Stefan&WARGNIES,%20Marion&PLAZOLLES,%20Nicolas&CAHOREAU,%20Edern&BIRAN,%20Marc&rft.genre=article


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