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Visualizing and profiling lipids in the OVLT of Fat-1 and wild type mouse brains during LPS-induced systemic inflammation using AP-SMALDI MSI

dc.rights.licenseopenen_US
dc.contributor.authorBREDEHOFT, Janne
dc.contributor.authorBHANDARI, Dhaka Ram
dc.contributor.authorPFLIEGER, Fabian Johannes
dc.contributor.authorSCHULZ, Sabine
dc.contributor.authorKANG, Jing X.
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorLAYE, Sophie
ORCID: 0000-0002-3843-1012
IDREF: 11366883X
dc.contributor.authorROTH, Joachim
dc.contributor.authorGERSTBERGER, Rüdiger
dc.contributor.authorMAYER, Konstantin
dc.contributor.authorSPENGLER, Bernhard
dc.contributor.authorRUMMEL, Christoph
dc.date.accessioned2021-09-22T14:24:40Z
dc.date.available2021-09-22T14:24:40Z
dc.date.issued2019
dc.identifier.issn1948-7193en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/112334
dc.description.abstractEnLipids, including omega-3 polyunsaturated fatty acids (n-3-PUFAs), modulate brain-intrinsic inflammation during systemic inflammation. The vascular organ of the lamina terminalis (OVLT) is a brain structure important for immune-to-brain communication. We, therefore, aimed to profile the distribution of several lipids (e.g., phosphatidyl-choline/ethanolamine, PC/PE), including n-3-PUFA-carrying lipids (esterified in phospholipids), in the OVLT during systemic lipopolysaccharide(LPS)-induced inflammation. We injected wild type and endogenously n-3-PUFA producing fat-1 transgenic mice with LPS (i.p., 2.5 mg/kg) or PBS. Brain samples were analyzed using immunohistochemistry and high-resolution atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization orbital trapping mass spectrometry imaging (AP-SMALDI-MSI) for spatial resolution of lipids. Depending on genotype and treatment, several distinct distribution patterns were observed for lipids [e.g., lyso(L)PC (16:0)/(18:0)] proposed to be involved in inflammation. The distribution patterns ranged from being homogeneously disseminated [LPC (18:1)], absent/reduced signaling within the OVLT relative to adjacent preoptic tissue [PE (38:6)], either treatment- and genotype-dependent or independent low signal intensities [LPC (18:0)], treatment- and genotype-dependent [PC 38:6)] or independent accumulation in the OVLT [PC (38:7)], and accumulation in commissures, e.g., nerve fibers like the optic nerve [LPE (18:1)]. Overall, screening of lipid distribution patterns revealed distinct inflammation-induced changes in the OVLT, highlighting the prominent role of lipid metabolism in brain inflammation. Moreover, known and novel candidates for brain inflammation and immune-to-brain communication were detected specifically within this pivotal brain structure, a window between the periphery and the brain. The biological significance of these newly identified lipids abundant in the OVLT and the adjacent preoptic area remains to be further analyzed.
dc.language.isoENen_US
dc.subjectMALDI imaging
dc.subjectVascular organ of laminae terminalis
dc.subjectLipopolysaccharide
dc.subjectImmune-to-brain communication
dc.subjectBrain lipids
dc.subjectOmega-3 polyunsaturated fatty acids
dc.title.enVisualizing and profiling lipids in the OVLT of Fat-1 and wild type mouse brains during LPS-induced systemic inflammation using AP-SMALDI MSI
dc.typeArticle de revueen_US
dc.identifier.doi10.1021/acschemneuro.9b00435en_US
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]en_US
dc.identifier.pubmed31513369en_US
bordeaux.journalACS Chemical Neuroscienceen_US
bordeaux.page4394-4406en_US
bordeaux.volume10en_US
bordeaux.hal.laboratoriesNutriNeurO (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286en_US
bordeaux.issue10en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINRAEen_US
bordeaux.teamPsychoneuroimmunologie et Nutrition: Approches expérimentales et cliniquesen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDDeutsche Forschungsgemeinschaften_US
hal.exportfalse
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=ACS%20Chemical%20Neuroscience&rft.date=2019&rft.volume=10&rft.issue=10&rft.spage=4394-4406&rft.epage=4394-4406&rft.eissn=1948-7193&rft.issn=1948-7193&rft.au=BREDEHOFT,%20Janne&BHANDARI,%20Dhaka%20Ram&PFLIEGER,%20Fabian%20Johannes&SCHULZ,%20Sabine&KANG,%20Jing%20X.&rft.genre=article


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