Afficher la notice abrégée

Implication of vitamin A in neuroprotection of dopaminergic neurons in a rat model of Parkinson's disease

dc.rights.licenseopenen_US
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorMARIE, Anais
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorLEROY, Julien
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorPALLET, Veronique
ORCID: 0000-0002-9078-3269
IDREF: 066841763
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorVANCASSEL, Sylvie
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorBOSCH BOUJU, Clementine
ORCID: 0000-0001-8869-768X
IDREF: 156530244
dc.date.accessioned2021-09-21T07:35:14Z
dc.date.available2021-09-21T07:35:14Z
dc.date.issued2019-05
dc.date.conference2019-05-22
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/112265
dc.descriptionPosteren_US
dc.description.abstractEnParkinson's disease (PD) is caused by a loss of dopaminergic neurons in the substantia nigra pars compacta (SNc), leading to strong motor impairments. Dopaminergic neurons from the SNc project to the striatum which allow the control of voluntary movements. Vitamin A, through the action of its active metabolite, retinoic acid (RA), is involved in the development, differentiation and neuroprotection of SNc dopaminergic neurons. Additionally, retinaldehyde dehydrogenase (RALDH), the synthesis enzyme of retinoic acid, is involved in cellular detoxification. However, the cerebral bioavailability of retinoic acid decreases with aging, which downregulates RALDH expression. This may precipitate neurodegenerative processes such as those observed in Parkinson's disease. Here we hypothesize that nutritional supplementation with vitamin A normalizes brain levels of retinoic acid and thus RALDH, which exhibit a neuroprotective effect on dopaminergic neurons, to delay the progression of the disease. To test our hypothesis, we modeled Parkinson's disease with unilateral injection of 6-hydroxydopamine (6-OHDA), a toxin that selectively destroys dopaminergic neurons, into rats' striatum. Rats were supplemented or not with dietary vitamin A (20 UI/g) for 5 weeks before the lesion. Motor impairments induced by 6-OHDA and protective effect of vitamin A were quantified with the step test, cylinder test and rotarod. Extend of dopaminergic neurons degeneration was assessed with stereological analysis of tyrosine hydroxylase, the production enzyme of dopamine, staining in the striatum. To evaluate the functionality of the dopaminergic system, levels of dopamine and its metabolites were measured in the striatum with HPLC. Finally, to precisely interrogate the impact of vitamin A supplementation on RALDH enzyme, its expression and localization will be assessed with immunostaining, western blot and RT-qPCR analyses. These experiments that are still in progress will allow a precise assessment of the neuroprotective effects of vitamin A supplementation on dopaminergic neurons in a rat model of Parkinson's disease. This work may open therapeutic strategies to prevent neurodegeneration.
dc.language.isoENen_US
dc.title.enImplication of vitamin A in neuroprotection of dopaminergic neurons in a rat model of Parkinson's disease
dc.typeAutre communication scientifique (congrès sans actes - poster - séminaire...)en_US
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]en_US
bordeaux.hal.laboratoriesNutriNeurO (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINRAEen_US
bordeaux.conference.titleColloque International NeuroFrance 2019en_US
bordeaux.countryfren_US
bordeaux.teamPsychoneuroimmunologie et Nutrition: Approches expérimentales et cliniquesen_US
bordeaux.teamNutrition, mémoire et glucocorticoïdesen_US
bordeaux.conference.cityMarseilleen_US
bordeaux.peerReviewedouien_US
hal.exportfalse
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2019-05&rft.au=MARIE,%20Anais&LEROY,%20Julien&PALLET,%20Veronique&VANCASSEL,%20Sylvie&BOSCH%20BOUJU,%20Clementine&rft.genre=conference


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée