PFEFFER, Marlene; PRONOT, Marie; ANGELO, Maria-Florencia; WALLE, Roman; CORDELIERES, Fabrice; LEVET, Florian; CLAVEROL, Stephane; LACOMME, Sabrina; PETREL, Melina; MARTIN, Christelle; PITARD, Vincent; DE SMEDT-PEYRUSSE, Veronique; BIEDERER, Thomas; TRIFILIEFF, Pierre; HERZOG, Etienne

doi:10.1101/2020.02.18.952978

Interdisciplinary Institute for Neuroscience / Institut interdisciplinaire de neurosciences [Bordeaux] [IINS]

PRONOT, Marie
Interdisciplinary Institute for Neuroscience / Institut interdisciplinaire de neurosciences [Bordeaux] [IINS]

ANGELO, Maria-Florencia
Interdisciplinary Institute for Neuroscience / Institut interdisciplinaire de neurosciences [Bordeaux] [IINS]

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Document de travail - Pré-publication

Este ítem está publicado en

2020

Resumen en inglés

Dopamine transmission is a monoaminergic system involved in reward processing and motor control. Volume transmission is thought to be the main mechanism by which monoamines modulate effector transmission though synaptic structures are scarcely described. Here, we applied a fluorescence activated synaptosome sorting workflow to dopaminergic projections to the striatum and explored cellular and molecular features of the dopaminergic synaptome. This demonstrated that dopaminergic varicosities adhere to post-synaptic membrane baring cognate receptors. We further identified a specific bond of varicosities to glutamatergic or GABAergic synapses in structures we named dopaminergic “hub synapses”. Finally, we showed that the synaptic adhesion protein SynCAM 2 is strongly expressed at dopaminergic hub synapses. Our data strongly suggest that neuromodulation frequently operates from hub-synapses on local receptors, presumably in conjunction with extra-synaptic volume transmission. We provide a new framework for the molecular exploration of dopaminergic synapses and more generally on discrete synapse populations ex-vivo.< Leer menos

URI

https://oskar-bordeaux.fr/handle/20.500.12278/112246

DOI

http://dx.doi.org/10.1101/2020.02.18.952978

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NutriNeuro (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286

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Synaptic and supra-synaptic organisation of the dopaminergic projection to the striatum

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Este ítem está publicado en

Resumen en inglés

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DOI

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