Mostrar el registro sencillo del ítem

Combined Gene Expression and Chromatin Immunoprecipitation From a Single Mouse Hippocampus

dc.rights.licenseopenen_US
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorHELBLING, Jean Christophe
dc.contributor.authorKINOUCHI, Kenichiro
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorTRIFILIEFF, Pierre
dc.contributor.authorSASSONE‐CORSI, Paolo
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorMOISAN, Marie Pierre
IDREF: 060242264
dc.date.accessioned2021-09-17T09:19:47Z
dc.date.available2021-09-17T09:19:47Z
dc.date.issued2021-02-01
dc.identifier.issn2691-1299en_US
dc.identifier.urioai:crossref.org:10.1002/cpz1.33
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/112229
dc.description.abstractEnAll neuronal cells hold the same genetic information but vary by their structural and functional plasticity depending on the brain area and environmental influences. Such variability involves specific gene regulation, which is driven by transcription factors (TFs). In the field of neuroscience, epigenetics is the main mechanism that has been investigated to understand the dynamic modulation of gene expression by behavioral responses, stress responses, memory processes, etc. Nowadays, gene expression analyzed by real-time quantitative PCR and TF binding estimated by chromatin immunoprecipitation (ChIP) enables one to dissect this regulation. Because of the wide range of transgenic models, as well as cost-effective aspects, mouse models are widely used neuroscience. Thus, we have set up a protocol that allows extraction of both RNA for gene expression analysis and chromatin for ChIP experiment from a single mouse hippocampus. Using such protocols, information regarding gene expression and regulatory molecular mechanisms from the same animal can be integrated and correlated with neurobiological and behavioral outcomes.
dc.language.isoENen_US
dc.sourcecrossref
dc.subject.enChIP
dc.subject.enEpigenetic
dc.subject.enGene expression
dc.subject.enHippocampus
dc.subject.enMouse
dc.title.enCombined Gene Expression and Chromatin Immunoprecipitation From a Single Mouse Hippocampus
dc.typeArticle de revueen_US
dc.identifier.doi10.1002/cpz1.33en_US
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]
dc.identifier.pubmed33566459en_US
bordeaux.journalCurrent Protocolsen_US
bordeaux.volume1en_US
bordeaux.hal.laboratoriesNutriNeurO (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286en_US
bordeaux.issue2en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINRAEen_US
bordeaux.teamPsychoneuroimmunologie et Nutrition: Approches expérimentales et cliniquesen_US
bordeaux.teamNutrition, mémoire et glucocorticoïdesen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.exportfalse
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Current%20Protocols&rft.date=2021-02-01&rft.volume=1&rft.issue=2&rft.eissn=2691-1299&rft.issn=2691-1299&rft.au=HELBLING,%20Jean%20Christophe&KINOUCHI,%20Kenichiro&TRIFILIEFF,%20Pierre&SASSONE%E2%80%90CORSI,%20Paolo&MOISAN,%20Marie%20Pierre&rft.genre=article


Archivos en el ítem

ArchivosTamañoFormatoVer

No hay archivos asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem