HELBLING, Jean Christophe; KINOUCHI, Kenichiro; TRIFILIEFF, Pierre; SASSONE‐CORSI, Paolo; MOISAN, Marie Pierre

doi:10.1002/cpz1.33

Metadatos

Mostrar el registro completo del ítem

Licencia de uso del documento

HELBLING, Jean Christophe
Nutrition et Neurobiologie intégrée [NutriNeuro]

KINOUCHI, Kenichiro

TRIFILIEFF, Pierre
Nutrition et Neurobiologie intégrée [NutriNeuro]

Idioma

Article de revue

Este ítem está publicado en

Current Protocols. 2021-02-01, vol. 1, n° 2

Resumen en inglés

All neuronal cells hold the same genetic information but vary by their structural and functional plasticity depending on the brain area and environmental influences. Such variability involves specific gene regulation, which is driven by transcription factors (TFs). In the field of neuroscience, epigenetics is the main mechanism that has been investigated to understand the dynamic modulation of gene expression by behavioral responses, stress responses, memory processes, etc. Nowadays, gene expression analyzed by real-time quantitative PCR and TF binding estimated by chromatin immunoprecipitation (ChIP) enables one to dissect this regulation. Because of the wide range of transgenic models, as well as cost-effective aspects, mouse models are widely used neuroscience. Thus, we have set up a protocol that allows extraction of both RNA for gene expression analysis and chromatin for ChIP experiment from a single mouse hippocampus. Using such protocols, information regarding gene expression and regulatory molecular mechanisms from the same animal can be integrated and correlated with neurobiological and behavioral outcomes.< Leer menos

Palabras clave en inglés

ChIP

Epigenetic

Gene expression

Hippocampus

Mouse

Metadatos

Compartir este ítem

Licencia de uso del documento

Combined Gene Expression and Chromatin Immunoprecipitation From a Single Mouse Hippocampus

Idioma

Este ítem está publicado en

Resumen en inglés

Palabras clave en inglés

URI

DOI

Centros de investigación