Mostrar el registro sencillo del ítem
Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier
| dc.rights.license | open | en_US |
| dc.contributor.author | ZHU, Ying | |
| dc.contributor.author | MESZAROS, Jozsef | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | WALLE, Roman | |
| dc.contributor.author | FAN, Rongxi | |
| dc.contributor.author | SUN, Ziyi | |
| dc.contributor.author | DWORK, Andrew J. | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | TRIFILIEFF, Pierre | |
| dc.contributor.author | JAVITCH, Jonathan A. | |
| dc.date.accessioned | 2021-08-31T10:22:18Z | |
| dc.date.available | 2021-08-31T10:22:18Z | |
| dc.date.issued | 2019-12-20 | |
| dc.identifier.issn | 1940-9818 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/110267 | |
| dc.description.abstractEn | Despite the controversy regarding the existence and physiological relevance of class A G protein-coupled receptor dimerization, there is substantial evidence for functional interactions between the dopamine D2 receptor (D2R) and the adenosine A2A receptor (A2AR). A2AR-D2R complexes have been detected in rodent brains by proximity ligation assay; however, their existence in the human brain has not been demonstrated. In this study, we used Brightfield proximity ligation assay, combined with a systematic sampling and a parameter-free naive Bayesian classifier, and demonstrated proximity between the D2R and the A2AR in the adult human ventral striatum, consistent with their colocalization within complexes and the possible existence of D2R-A2AR heteromers. These methods are applicable to the relative quantification of proximity of two proteins, as well as the expression levels of individual proteins. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
| dc.subject.en | Adenosine | |
| dc.subject.en | Adenosine A2A receptor | |
| dc.subject.en | Dopamine | |
| dc.subject.en | Dopamine receptor type 2 | |
| dc.subject.en | G protein-coupled receptor | |
| dc.subject.en | Machine learning | |
| dc.subject.en | Naive Bayesian classifier | |
| dc.subject.en | Proximity ligation assay | |
| dc.title.en | Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.2144/btn-2019-0083 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC] | en_US |
| dc.identifier.pubmed | 31859535 | en_US |
| bordeaux.journal | Biotechniques | en_US |
| bordeaux.page | 122-129 | en_US |
| bordeaux.volume | 68 | en_US |
| bordeaux.hal.laboratories | NutriNeurO (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286 | en_US |
| bordeaux.issue | 3 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INRAE | en_US |
| bordeaux.team | Psychoneuroimmunologie et Nutrition: Approches expérimentales et cliniques | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-03329843 | |
| hal.version | 1 | |
| hal.date.transferred | 2021-08-31T10:22:23Z | |
| hal.export | true | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biotechniques&rft.date=2019-12-20&rft.volume=68&rft.issue=3&rft.spage=122-129&rft.epage=122-129&rft.eissn=1940-9818&rft.issn=1940-9818&rft.au=ZHU,%20Ying&MESZAROS,%20Jozsef&WALLE,%20Roman&FAN,%20Rongxi&SUN,%20Ziyi&rft.genre=article |
