Afficher la notice abrégée

Recombinant production and purification of short hydrophobic Elastin-like polypeptides with low transition temperatures

dc.contributor.authorBATAILLE, Laure
dc.contributor.authorDIERYCK, Wilfrid
dc.contributor.authorHOCQUELLET, Agnes
dc.contributor.authorCABANNE, Charlotte
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBATHANY, Katell
dc.contributor.authorLECOMMANDOUX, Sebastien
dc.contributor.authorGARBAY, Bertrand
ORCID: 0000-0001-5756-2627
IDREF: 033777551
dc.contributor.authorGARANGER, Elisabeth
IDREF: 089451740
dc.date.accessioned2020-09-03T08:02:20Z
dc.date.available2020-09-03T08:02:20Z
dc.date.issued2015
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/11017
dc.description.abstractEnElastin-like polypeptides (ELPs) are biodegradable polymers with interesting physico-chemical properties for biomedical and biotechnological applications. We report herein the recombinant expression of three hydrophobic ELPs (VPGIG)n with variable lengths (n=20, 40, 60) and sub-ambient transition temperatures. These ELPs were purified from the cytoplasmic soluble fraction of Escherichia coli by inverse transition cycling, and their exact molecular weight was confirmed by various mass spectrometry techniques. Transition temperatures of ELP20, ELP40, and ELP60 were measured at 18.6°C, 12.4°C and 11.7°C, respectively.
dc.language.isoen
dc.title.enRecombinant production and purification of short hydrophobic Elastin-like polypeptides with low transition temperatures
dc.typeArticle de revue
dc.identifier.doi10.1016/j.pep.2016.01.010
dc.subject.halChimie/Matériaux
bordeaux.journalProtein Expression and Purification
bordeaux.page81-7
bordeaux.volume121
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Protein%20Expression%20and%20Purification&rft.date=2015&rft.volume=121&rft.spage=81-7&rft.epage=81-7&rft.au=BATAILLE,%20Laure&DIERYCK,%20Wilfrid&HOCQUELLET,%20Agnes&CABANNE,%20Charlotte&BATHANY,%20Katell&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée