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The Qb-SNARE Memb11 interacts specifically with Arf1 in the Golgi apparatus of Arabidopsis thaliana

dc.contributor.authorMARAIS, C.
dc.contributor.authorWATTELET-BOYER, V.
dc.contributor.authorBOUYSSOU, G.
dc.contributor.authorHOCQUELLET, Agnes
dc.contributor.authorDUPUY, J. W.
dc.contributor.authorBATAILLER, B.
dc.contributor.authorBROCARD, L.
dc.contributor.authorBOUTTE, Yohann
dc.contributor.authorMANETA-PEYRET, Lilly
dc.contributor.authorMOREAU, Patrick
IDREF: 058610723
dc.date.accessioned2020-09-03T08:02:03Z
dc.date.available2020-09-03T08:02:03Z
dc.date.issued2015
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10958
dc.description.abstractEnThe SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins are critical for the function of the secretory pathway. The SNARE Memb11 is involved in membrane trafficking at the ER-Golgi interface. The aim of the work was to decipher molecular mechanisms acting in Memb11-mediated ER-Golgi traffic. In mammalian cells, the orthologue of Memb11 (membrin) is potentially involved in the recruitment of the GTPase Arf1 at the Golgi membrane. However molecular mechanisms associated to Memb11 remain unknown in plants. Memb11 was detected mainly at the cis-Golgi and co-immunoprecipitated with Arf1, suggesting that Arf1 may interact with Memb11. This interaction of Memb11 with Arf1 at the Golgi was confirmed by in vivo BiFC (Bimolecular Fluorescence Complementation) experiments. This interaction was found to be specific to Memb11 as compared to either Memb12 or Sec22. Using a structural bioinformatic approach, several sequences in the N-ter part of Memb11 were hypothesized to be critical for this interaction and were tested by BiFC on corresponding mutants. Finally, by using both in vitro and in vivo approaches, we determined that only the GDP-bound form of Arf1 interacts with Memb11. Together, our results indicate that Memb11 interacts with the GDP-bound form of Arf1 in the Golgi apparatus. © 2015 The Author.
dc.language.isoen
dc.title.enThe Qb-SNARE Memb11 interacts specifically with Arf1 in the Golgi apparatus of Arabidopsis thaliana
dc.typeArticle de revue
dc.identifier.doi10.1093/jxb/erv373
dc.subject.halChimie/Matériaux
bordeaux.journalJournal of Experimental Botany
bordeaux.page6665-6678
bordeaux.volume66
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue21
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
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