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Quaternary ammonium groups exposed at the surface of silica nanoparticles suitable for DNA complexation in the presence of cationic lipids

dc.contributor.authorREINHARDT, Nora
dc.contributor.authorADUMEAU, Laurent
dc.contributor.authorLAMBERT, Olivier
dc.contributor.authorRAVAINE, Serge
dc.contributor.authorMORNET, Stephane
dc.date.accessioned2020-09-03T08:01:56Z
dc.date.available2020-09-03T08:01:56Z
dc.date.issued2015
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10931
dc.description.abstractEnThe production of silica nanoparticles (NPs) exposing quaternary ammonium groups (NPQ(+)) has been achieved using an optimized chemical surface functionalization protocol. The procedures of surface modification and quaternization of amino groups were validated by diffuse reflectance infrared Fourier transform (DRIFT) and (1)H NMR spectroscopies. Compared to nonquaternized aminated NP, the colloidal stability of NPQ(+) was improved for various pH and salt conditions as assessed by zeta potential and light scattering measurements. In the context of their use for nucleic acid delivery, DNA efficiently bound to NPQ(+) analyzed by cosedimentation assays for a large pH range and various NaCl concentrations and exhibited a better efficacy at basic pH than nonquaternized NP. The study of NPQ(+)/DNA/cationic lipids ternary complexes was carried out with 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and analyzed by cryo-electron microscopy (cryo-EM). Cryo-EM images showed ternary assemblies where condensed DNA strands are sandwiched between the NPQ(+) surface and the cationic lipid bilayer. Because of an unusual electrostatic colloidal stability of NPQ(+) and a high propensity to bind DNA molecules particularly at high salt concentrations, a novel type of ternary assembly has been formed that might impact the delivery properties of these complexes including their stability in biological environment.
dc.language.isoen
dc.title.enQuaternary ammonium groups exposed at the surface of silica nanoparticles suitable for DNA complexation in the presence of cationic lipids
dc.typeArticle de revue
dc.identifier.doi10.1021/acs.jpcb.5b01834
dc.subject.halChimie/Matériaux
bordeaux.journalThe journal of physical chemistry. B
bordeaux.page6401-11
bordeaux.volume119
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue21
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=The%20journal%20of%20physical%20chemistry.%20B&rft.date=2015&rft.volume=119&rft.issue=21&rft.spage=6401-11&rft.epage=6401-11&rft.au=REINHARDT,%20Nora&ADUMEAU,%20Laurent&LAMBERT,%20Olivier&RAVAINE,%20Serge&MORNET,%20Stephane&rft.genre=article


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