Show simple item record

dc.rights.licenseopenen_US
dc.contributor.authorPOLI, Evelyne
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorOUK, Tan-Sothea
dc.contributor.authorBARRIERE, Guislaine
dc.contributor.authorLEVEQUE, Guillaume
dc.contributor.authorSOL, Vincent
dc.contributor.authorDENES, Eric
dc.date.accessioned2021-07-16T14:31:18Z
dc.date.available2021-07-16T14:31:18Z
dc.date.issued2019
dc.identifier.issn1877-0568en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/109295
dc.description.abstractEnBackground: Bacterial adhesion depends on surface materials. Recently it was suggested that ceramic-on-ceramic bearings could be less prone to infection than other bearings. We examined the possibility that porous alumina ceramic could be less susceptible to bacterial adhesion. Hypothesis: As hydroxyl groups (OH) on material surface are a major factor governing the surface properties (for example: adsorption, first non-specific step of bacterial adhesion), we hypothesized that alumina had lower OH group density than other material. Thus, we asked (i) if bacterial adhesion was lower on alumina than on titanium alloy, stainless steel and polyethylene and (ii) if OH group density was also lower on alumina. Material and methods: We performed (i) in vitro bacterial cultures on porous alumina, titanium, stainless steel and polyethylene using Staphylococcus aureus and Pseudomonas aeruginosa, known to adhere to surfaces. Bacterial cultures were done 3 times in duplicate for each material and each strain. Colony Forming Units (CFU) per cm(2) were measured; (ii) Neutral red reagent helped obtaining OH density estimates using spacer arms. UV-visible spectrophotometry method with Neutral red test, reproduced twice for each surface, provided mu g/cm(2) measurements of OH density. Results: There was significantly less P. aeruginosa adherent on porous alumina (2.25x10(4) CFU/cm(2)) than on titanium (4.27x10(5) CFU/cm(2), p=0.01), on stainless steel (2.44x10(5) CFU/cm(2), p=0.02) and on polyethylene (7.29x10(5) CFU/cm(2), p<0.001). S. aureus was significantly less adherent on porous alumina (3.22x10(5) CFU/cm(2)) than on polyethylene (5.23x10(6) CFU/cm(2), p=0.01), but there was no difference with titanium (1.64x10(6) CFU/cm(2), p=0.08) and stainless steel (1.79x10(6) CFU/cm(2), p=0.1). There was significantly lower Neutral red grafted on porous alumina (0.09 mu g/cm(2)) than on titanium (8.88 mu g/cm(2), p<0.0001), on stainless steel (39.8 mu g/cm(2), p=0.002) and on polyethylene (4.5 mu g/cm(2), p<0.01). However, no correlation was found between bacterial adherence and OH group density. Discussion: Bacterial adherence on porous alumina was lower than on other bearings. Although there were less surface OH groups on porous alumina, we failed establishing a statistical correlation between bacterial adherence and OH group density. (C) 2018 Elsevier Masson SAS. All rights reserved.
dc.language.isoENen_US
dc.subject.enAlumina
dc.subject.enHydroxyl group
dc.subject.enAdhesion
dc.subject.enCeramic
dc.subject.enBacteria
dc.title.enDoes low hydroxyl group surface density explain less bacterial adhesion on porous alumina?
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.otsr.2018.11.018en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalOrthopaedics & Traumatology-Surgery & Researchen_US
bordeaux.page473-477en_US
bordeaux.volume105en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248en_US
bordeaux.issue3en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02895581
hal.exportfalse
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Orthopaedics%20&%20Traumatology-Surgery%20&%20Research&amp;rft.date=2019&amp;rft.volume=105&amp;rft.issue=3&amp;rft.spage=473-477&amp;rft.epage=473-477&amp;rft.eissn=1877-0568&amp;rft.issn=1877-0568&amp;rft.au=POLI,%20Evelyne&amp;OUK,%20Tan-Sothea&amp;BARRIERE,%20Guislaine&amp;LEVEQUE,%20Guillaume&amp;SOL,%20Vincent&amp;rft.genre=article


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record