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Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids

hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorCACAS, Jean-Luc
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBURE, Corinne
dc.contributor.authorGROSJEAN, Kevin
dc.contributor.authorGERBEAU-PISSOT, Patricia
dc.contributor.authorLHERMINIER, Jeannine
dc.contributor.authorROMBOUTS, Yoann
dc.contributor.authorMAES, Emmanuel
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorBOSSARD, Claire
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorGRONNIER, Julien
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorFURT, Fabienne
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorFOUILLEN, Laetitia
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorGERMAIN, Veronique
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorBAYER, Emmanuelle
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorCLUZET, Stephanie
dc.contributor.authorROBERT, Franck
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorSCHMITTER, Jean-Marie
dc.contributor.authorDELEU, Magali
dc.contributor.authorLINS, Laurence
dc.contributor.authorSIMON-PLAS, Francoise
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorMONGRAND, Sébastien
dc.date.accessioned2020-09-03T07:56:26Z
dc.date.available2020-09-03T07:56:26Z
dc.date.issued2016
dc.identifier.issn1532-2548
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10899
dc.description.abstractEnThe lipid composition of plasma membrane (PM) and the corresponding detergent-insoluble membrane (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides (GIPCs). Using tobacco (Nicotiana tabacum) 'Bright Yellow 2' cell suspension and leaves, evidence is provided that GIPCs represent up to 40 mol % of the PM lipids. Comparative analysis of DIMs with the PM showed an enrichment of 2-hydroxylated very-long-chain fatty acid-containing GIPCs and polyglycosylated GIPCs in the DIMs. Purified antibodies raised against these GIPCs were further used for immunogold-electron microscopy strategy, revealing the distribution of polyglycosylated GIPCs in domains of 35 ± 7 nm in the plane of the PM. Biophysical studies also showed strong interactions between GIPCs and sterols and suggested a role for very-long-chain fatty acids in the interdigitation between the two PM-composing monolayers. The ins and outs of lipid asymmetry, raft formation, and interdigitation in plant membrane biology are finally discussed. 2016 American Society of Plant Biologists. All Rights Reserved.
dc.language.isoen
dc.title.enRevisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids
dc.typeArticle de revue
dc.identifier.doi10.1104/pp.15.00564
dc.subject.halChimie/Matériaux
bordeaux.journalPlant physiology
bordeaux.page367-84
bordeaux.volume170
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue1
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.institutionINRAE
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20physiology&rft.date=2016&rft.volume=170&rft.issue=1&rft.spage=367-84&rft.epage=367-84&rft.eissn=1532-2548&rft.issn=1532-2548&rft.au=CACAS,%20Jean-Luc&BURE,%20Corinne&GROSJEAN,%20Kevin&GERBEAU-PISSOT,%20Patricia&LHERMINIER,%20Jeannine&rft.genre=article


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