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Water-in-oil microemulsions versus emulsions as carriers of hydroxytyrosol: an in vitro gastrointestinal lipolysis study using the pHstat technique

dc.relation.isnodouble8d481905-1286-49a7-a15c-81d178280e84*
dc.contributor.authorCHATZIDAKI, M. D.
dc.contributor.authorMATEOS-DIAZ, E.
dc.contributor.authorLEAL-CALDERON, F.
dc.contributor.authorXENAKIS, A.
dc.contributor.authorCARRIERE, F.
dc.date.accessioned2020-09-03T07:56:24Z
dc.date.available2020-09-03T07:56:24Z
dc.date.issued2016
dc.identifier.issn2042-6496
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10891
dc.description.abstractEnWater-in-oil (W/O) microemulsions and emulsions based on medium chain triglycerides (MCT) were successfully formulated with the addition of emulsifiers and used as encapsulation matrices for hydroxytyrosol (HT), an antioxidant naturally found in extra virgin olive oil. The digestibility of these edible W/O dispersions by recombinant dog gastric lipase (rDGL) and porcine pancreatic lipase (PPL) was then tested at different pH values using a pHstat device. rDGL and PPL displayed a much lower activity on the W/O microemulsion than that on the W/O emulsion and MCT alone. This was explained by the presence of higher amounts of emulsifiers (4.9% w/w lecithin and monoglycerides) in the composition of W/O microemulsions compared to W/O emulsions (1.3% w/w emulsifiers). These surfactants also induced a shift of maximum lipase activity towards lower pH values, which usually reflects the competition between surfactants and lipases for binding at the lipid-water interface. rDGL and PPL were then used consecutively in a two-step digestion model mimicking the conditions found in the human gastrointestinal tract. Direct titration and back-titration of free fatty acids allowed the continuous estimation of lipolysis rates under both gastric and duodenal conditions. Gastric lipolysis of W/O microemulsions was reduced 6 to 9-fold compared to W/O emulsions. This inhibition had a major impact on the overall lipolysis, although duodenal lipolysis was less affected by the dispersion type. The presence of HT had also some minor effects on lipolysis rates.
dc.language.isoen
dc.title.enWater-in-oil microemulsions versus emulsions as carriers of hydroxytyrosol: an in vitro gastrointestinal lipolysis study using the pHstat technique
dc.typeArticle de revue
dc.identifier.doi10.1039/c6fo00361c
dc.subject.halChimie/Matériaux
bordeaux.journalFood & Function
bordeaux.page2258-2269
bordeaux.volume7
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue5
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
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