Sequestration of Proteins by Fatty Acid Coacervates for Their Encapsulation within Vesicles
| dc.contributor.author | GARENNE, David | |
| dc.contributor.author | BEVEN, Laure
IDREF: 126206244 | |
| dc.contributor.author | NAVAILLES, Laurence | |
| dc.contributor.author | NALLET, Frederic
IDREF: 068545487 | |
| dc.contributor.author | DUFOURC, Erick J. | |
| dc.contributor.author | DOULIEZ, Jean-Paul | |
| dc.date.accessioned | 2020-09-03T07:56:18Z | |
| dc.date.available | 2020-09-03T07:56:18Z | |
| dc.date.issued | 2016 | |
| dc.identifier.issn | 1433-7851 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/10870 | |
| dc.description.abstractEn | Encapsulating biological materials in lipid vesicles is of interest for mimicking cells; however, except in some particular cases, such processes do not occur spontaneously. Herein, we developed a simple and robust method for encapsulating proteins in fatty acid vesicles in high yields. Fatty acid based, membrane-free coacervates spontaneously sequester proteins and can reversibly form membranous vesicles upon varying the pHvalue, the precrowding feature in coacervates allowing for protein encapsulation within vesicles. We then produced enzyme-enriched vesicles and show that enzymatic reactions can occur in these micrometric capsules. This work could be of interest in the field of synthetic biology for building microreactors. | |
| dc.language.iso | en | |
| dc.title.en | Sequestration of Proteins by Fatty Acid Coacervates for Their Encapsulation within Vesicles | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1002/anie.201607117 | |
| dc.subject.hal | Chimie/Matériaux | |
| bordeaux.journal | Angewandte Chemie-International Edition | |
| bordeaux.page | 13475-13479 | |
| bordeaux.volume | 55 | |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | * |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248) | |
| bordeaux.issue | 43 | |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | Bordeaux INP | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Angewandte%20Chemie-International%20Edition&rft.date=2016&rft.volume=55&rft.issue=43&rft.spage=13475-13479&rft.epage=13475-13479&rft.eissn=1433-7851&rft.issn=1433-7851&rft.au=GARENNE,%20David&BEVEN,%20Laure&NAVAILLES,%20Laurence&NALLET,%20Frederic&DUFOURC,%20Erick%20J.&rft.genre=article |
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