GARENNE, David; BEVEN, Laure; NAVAILLES, Laurence; NALLET, Frederic; DUFOURC, Erick J.; DOULIEZ, Jean-Paul

doi:10.1002/anie.201607117

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Angewandte Chemie-International Edition. 2016, vol. 55, n° 43, p. 13475-13479

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Encapsulating biological materials in lipid vesicles is of interest for mimicking cells; however, except in some particular cases, such processes do not occur spontaneously. Herein, we developed a simple and robust method for encapsulating proteins in fatty acid vesicles in high yields. Fatty acid based, membrane-free coacervates spontaneously sequester proteins and can reversibly form membranous vesicles upon varying the pHvalue, the precrowding feature in coacervates allowing for protein encapsulation within vesicles. We then produced enzyme-enriched vesicles and show that enzymatic reactions can occur in these micrometric capsules. This work could be of interest in the field of synthetic biology for building microreactors.< Leer menos

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Sequestration of Proteins by Fatty Acid Coacervates for Their Encapsulation within Vesicles

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