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Structure elucidation of the Pribnow box consensus promoter sequence by racemic DNA crystallography

dc.contributor.authorMANDAL, Pradeep K.
dc.contributor.authorCOLLIE, Gavin W.
dc.contributor.authorSRIVASTAVA, Suresh C.
dc.contributor.authorKAUFFMANN, Brice
dc.contributor.authorHUC, Ivan
dc.date.accessioned2020-09-03T07:56:11Z
dc.date.available2020-09-03T07:56:11Z
dc.date.issued2016
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10842
dc.description.abstractEnIt has previously been shown that the use of racemic mixtures of naturally chiral macromolecules such as protein and DNA can significantly aid the crystallogenesis process, thereby addressing one of the major bottlenecks to structure determination by X-ray crystallographic methods-that of crystal growth. Although previous studies have provided convincing evidence of the applicability of the racemic crystallization technique to DNA through the study of well-characterized DNA structures, we sought to apply this method to a historically challenging DNA sequence. For this purpose we chose a non-self-complementary DNA duplex containing the biologically-relevant Pribnow box consensus sequence 'TATAAT'. Four racemic crystal structures of this previously un-crystallizable DNA target are reported (with resolutions in the range of 1.65-2.3 A), with further crystallographic studies and structural analysis providing insight into the racemic crystallization process as well as structural details of this highly pertinent DNA sequence.
dc.language.isoen
dc.title.enStructure elucidation of the Pribnow box consensus promoter sequence by racemic DNA crystallography
dc.typeArticle de revue
dc.identifier.doi10.1093/nar/gkw367
dc.subject.halChimie/Matériaux
bordeaux.journalNucleic acids research
bordeaux.page5936-43
bordeaux.volume44
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue12
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nucleic%20acids%20research&rft.date=2016&rft.volume=44&rft.issue=12&rft.spage=5936-43&rft.epage=5936-43&rft.au=MANDAL,%20Pradeep%20K.&COLLIE,%20Gavin%20W.&SRIVASTAVA,%20Suresh%20C.&KAUFFMANN,%20Brice&HUC,%20Ivan&rft.genre=article


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