CABANNE, Charlotte; SANTARELLI, Xavier

doi:10.2174/1389203718666171030104211

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CABANNE, Charlotte

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

SANTARELLI, Xavier

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

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Current protein & peptide science. 2019, vol. 20, n° 1, p. 22-27

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Mixed mode chromatography resins with salt tolerance, large design space and orthogonal selectivity requires a slightly more complex development than traditional resins. It is important to screen several ligands and several binding and elution conditions. This allows taking full advantage of these resins. High-Throughput Screening (HTS) for Process Development should be done with the help of Design of Experiment (DoE). It could be performed in filter plates or Robocolumns, and assisted by liquid handling automated workstation. Modeling of the results allows the choice of optimal parameters that can then be validated and scaled up. All this leads to a better knowledge and robustness of the purification step.< Leer menos

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Mixed mode chromatography

high throughput process development

design of experiment

large opportunities

High-Throughput Screening (HTS)

Design of Experiment (DoE)

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Mixed Mode Chromatography, Complex Development for Large Opportunities

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