Mixed Mode Chromatography, Complex Development for Large Opportunities
Language
EN
Article de revue
This item was published in
Current protein & peptide science. 2019, vol. 20, n° 1, p. 22-27
English Abstract
Mixed mode chromatography resins with salt tolerance, large design space and orthogonal selectivity requires a slightly more complex development than traditional resins. It is important to screen several ligands and several ...Read more >
Mixed mode chromatography resins with salt tolerance, large design space and orthogonal selectivity requires a slightly more complex development than traditional resins. It is important to screen several ligands and several binding and elution conditions. This allows taking full advantage of these resins. High-Throughput Screening (HTS) for Process Development should be done with the help of Design of Experiment (DoE). It could be performed in filter plates or Robocolumns, and assisted by liquid handling automated workstation. Modeling of the results allows the choice of optimal parameters that can then be validated and scaled up. All this leads to a better knowledge and robustness of the purification step.Read less <
English Keywords
Mixed mode chromatography
high throughput process development
design of experiment
large opportunities
High-Throughput Screening (HTS)
Design of Experiment (DoE)