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Provir/Latitude 45 study: A step towards a multi-epitopic CTL vaccine designed on archived HIV-1 DNA and according to dominant HLA I alleles

dc.rights.licenseopenen_US
dc.contributor.authorTUMIOTTO, C.
dc.contributor.authorALVES, B. M.
dc.contributor.authorRECORDON-PINSON, P.
dc.contributor.authorJOURDAIN, M.
dc.contributor.authorBELLECAVE, P.
dc.contributor.authorGUIDICELLI, G. L.
dc.contributor.authorVISENTIN, J.
hal.structure.identifierBordeaux population health [BPH]
dc.contributor.authorBONNET, Fabrice
dc.contributor.authorHESSAMFAR, M.
dc.contributor.authorNEAU, D.
dc.contributor.authorSANCHEZ, J.
dc.contributor.authorBRANDER, C.
dc.contributor.authorSAJADI, M.
dc.contributor.authorEYZAGUIRRE, L.
dc.contributor.authorSOARES, E. A.
dc.contributor.authorROUTY, J. P.
dc.contributor.authorSOARES, M. A.
dc.contributor.authorFLEURY, H.
dc.date.accessioned2020-07-16T10:05:31Z
dc.date.available2020-07-16T10:05:31Z
dc.date.issued2019-02
dc.identifier.issn1932-6203 (Electronic) 1932-6203 (Linking)en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10488
dc.description.abstractEnOne of the approaches by which the scientific community is seeking to cure HIV is the use of therapeutic vaccination. Previous studies have highlighted the importance of the virus-specific CD8+ T cell cytotoxic responses for the immune control of HIV and have oriented research on vaccine constructs based on CTL epitopes from circulating HIV-1 strains. The clinical trials with therapeutic vaccines to date have had limited success likely due to (i) a discrepancy between archived CTL epitopes in the viral reservoir and those in circulating viruses before antiretroviral therapy (ART) initiation and (ii) the lack of strong affinity between the selected CTL epitopes and the HLA grooves for presentation to CD8+ cells. To overcome these limitations, we launched the Provir/Latitude 45 study to identify conserved CTL epitopes in archived HIV-1 DNA according to the HLA class I alleles of aviremic patients, most of whom are under ART. The near full-length genomes or Gag, Pol and Nef regions of proviral DNA were sequenced by Sanger and/or Next Generation Sequencing (NGS). The HLA-A and B alleles were defined by NGS or molecular analysis. The TuTuGenetics software, which moves a sliding window of 8 to 10 amino acids through the amino acid alignment, was combined with the Immune Epitope Data Base (IEDB) to automatically calculate the theoretical binding affinity of identified epitopes to the HLA alleles for each individual. We identified 15 conserved epitopes in Pol (11), Gag (3), and Nef (1) according to their potential presentation by the dominant HLA-A and B alleles and now propose to use the corresponding conserved peptides in a multi-epitopic vaccine (HLA-fitted VAC, HFVAC).
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/us/
dc.subject.enMORPH3Eus
dc.title.enProvir/Latitude 45 study: A step towards a multi-epitopic CTL vaccine designed on archived HIV-1 DNA and according to dominant HLA I alleles
dc.title.alternativePlos Oneen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1371/journal.pone.0212347en_US
dc.subject.halSciences du Vivant [q-bio]/Santé publique et épidémiologieen_US
dc.identifier.pubmed30811489en_US
bordeaux.journalPLoS ONEen_US
bordeaux.pagee0212347en_US
bordeaux.volume14en_US
bordeaux.hal.laboratoriesBordeaux Population Health Research Center (BPH) - U1219en_US
bordeaux.issue2en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02900644
hal.version1
hal.date.transferred2020-07-16T10:05:37Z
hal.exporttrue
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