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Hetero-oligomerization between the TNF receptor superfamily members CD40, Fas and TRAILR2 modulate CD40 signalling

dc.contributor.authorSMULSKI, Cristian R.
dc.contributor.authorDECOSSAS, Marion
dc.contributor.authorCHEKKAT, Neila
dc.contributor.authorBEYRATH, Julien
dc.contributor.authorWILLEN, Laure
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorGUICHARD, Gilles
IDREF: 084339268
dc.contributor.authorLORENZETTI, Raquel
dc.contributor.authorRIZZI, Marta
dc.contributor.authorEIBEL, Hermann
dc.contributor.authorSCHNEIDER, Pascal
dc.contributor.authorFOURNEL, Sylvie
dc.date.accessioned2020-07-09T14:16:55Z
dc.date.available2020-07-09T14:16:55Z
dc.date.issued2017-02
dc.identifier.issn2041-4889
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10323
dc.description.abstractEnTNF receptor superfamily members (TNFRSF) such as CD40, Fas and TRAIL receptor 2 (TRAILR2) participate to the adaptive immune response by eliciting survival, proliferation, differentiation and/or cell death signals. The balance between these signals determines the fate of the immune response. It was previously reported that these receptors are able to self-assemble in the absence of ligand through their extracellular regions. However, the role of this oligomerization is not well understood, and none of the proposed hypotheses take into account potential hetero-association of receptors. Using CD40 as bait in a flow cytometry Forster resonance energy transfer assay, TNFRSF members with known functions in B cells were probed for interactions. Both Fas and TRAILR2 associated with CD40. Immunoprecipitation experiments confirmed the interaction of CD40 with Fas at the endogenous levels in a BJAB B-cell lymphoma cell line deficient for TRAILR2. TRAILR2-expressing BJAB cells displayed a robust CD40-TRAILR2 interaction at the expense of the CD40-Fas interaction. The same results were obtained by proximity ligation assay, using TRAILR2-positive and -negative BJAB cells and primary human B cells. Expression of the extracellular domains of Fas or TRAILR2 with a glycolipid membrane anchor specifically reduced the intrinsic signalling pathway of CD40 in 293T cells. Conversely, BJAB cells lacking endogenous Fas or TRAILR2 showed an increased NF-kappa B response to CD40L. Finally, upregulation of TRAILR2 in primary human B cells correlated with reduced NF-kappa B activation and reduced proliferation in response to CD40L. Altogether, these data reveal that selective interactions between different TNFRSF members may modulate ligand-induced responses upstream signalling events.
dc.title.enHetero-oligomerization between the TNF receptor superfamily members CD40, Fas and TRAILR2 modulate CD40 signalling
dc.typeArticle de revue
dc.identifier.doi10.1038/cddis.2017.22
dc.subject.halChimie/Matériaux
bordeaux.journalCell death & disease
bordeaux.volume8
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
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